几种农用抗生素诱导植物对灰霉病的抗性及其信号转导途径研究

Analysis of agricultural antibiotics effects on plant resistance to gray mold and their signal transduction pathways

为探索申嗪霉素、多抗霉素、春雷霉素等3种农用抗生素在诱导植物灰霉病抗性中的作用,本试验通过温室条件下对烟草等植物叶片喷雾后,再用离体叶片接种灰霉病菌的方法检测了这3种抗生素在不同植物上对灰霉病的诱抗效果。另外,采用荧光定量PCR技术检测了3种抗生素诱抗处理后,烟草植株抗病信号转导途径中相关基因NPR1、CTR1、ETR1表达量的变化。结果表明:分别使用申嗪霉素40 mg/L 4 d、多抗霉素5 mg/L 4 d、春雷霉素80 mg/L 3 d诱导处理本氏烟、茄子、番茄、辣椒、黄瓜、草莓等不同植物后,再离体叶片接种灰霉病菌的病斑直径减小了25.86%~55.56%、25.17%~45.41%、20.17%~51.26%。同时,喷施3种抗生素均可激发烟草系统抗病标志基因NPR1的表达,激活SA和JA/ET信号转导途径,并抑制乙烯(ET)信号转导通路中负调控基因CTR1和ETR1的表达,从而诱导烟草植株产生系统抗病性。

In order to explore the role of shenqinmycin,polyoxin and chunleimycin in plant resistance to gray mold,the antibiotics were sprayed on the leaves of different plants followed by inoculating of Botrytis cinerea on detached leaves of tobacco,eggplant,tomato,pepper,cucumber,strawberry.Then the expression of NPR1,CTR1,ETR1 in signal transduction pathway were detected in tobacco leaves after treatments.The phenotypic observation showed that the lesion diameter of all the detached leaves reduced under treatments of shenzinomycin 40 mg/L for 4 d,polyoxin 5 mg/L for 4 d,and kasugamycin 80 mg/L for 3 d.Meanwhile,The three agricultural antibiotics stimulated the expression of disease-resistant marker gene NPR1 to activate the SA and JA/ET signal transduction pathway.and inhibitd the negative regulator of CTR1 gene and ETR1 gene in ethylene signaling,indicating the activation of systemic disease resistance of tobacco plants byantibiotics.