表告依春调控NF-kB相关炎症通路对骨肉瘤细胞恶性行为的抑制

Inhibitory Effect of Epigoitrin on Malignant Behavior of Osteosarcoma Cells Via Regulating NF-kB Related Inflammatory Pathway

目的探究表告依春(epigoitrin,EPG)对骨肉瘤细胞恶性行为的影响及相关机制。方法采用0、200、400及800μmol·L^(-1) EPG处理人成骨细胞HOB 24 h作为正常0、200、400和800μmol·L^(-1)组,0、200、400和800μmol·L^(-1) EPG处理骨肉瘤细胞U2OS 24 h作为肿瘤0、200、400和800μmol·L^(-1)组。Western blot实验检测细胞核因子-kB(nuclear factor kappa-B,NF-kB)、白介素-1β(interleukin-1β,IL-1β)及肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的表达,CCK-8实验检测细胞增殖能力,Transwell实验检测细胞侵袭及迁移能力。结果相比正常0μmol·L^(-1)组,正常800μmol·L^(-1)组NF-kB、IL-1β和TNF-α表达均降低(P均<0.01);相比肿瘤0μmol·L^(-1)组,肿瘤400、800μmol·L^(-1)组NF-kB、IL-1β及TNF-α表达均降低(P均<0.01),肿瘤200、400和800μmol·L^(-1)组细胞增殖、侵袭及迁移能力均减弱(P均<0.01)。结论EPG可下调炎症因子的表达,并抑制骨肉瘤细胞恶性行为。

Objective To investigate the effect of epigoitrin(EPG)on the malignant behavior of osteosarcoma cells and its mechanism.Methods Human osteoblasts HOB were treated with 0,200,400 and 800μmol·L^(-1) EPG for 24 h as normal 0,200,400,800μmol·L^(-1) groups,and osteosarcoma cells U2OS treated with 0,200,400 and 800μmol·L^(-1) EPG for 24 h were used as tumor 0,200,400,800μmol·L^(-1) groups.Western blot was used to detect the expression of nuclear factor kappa-B(NF-kB),interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α).CCK-8 assay was used to detect the proliferation of the cells.Transwell assay was used to detect the invasion and migration of the cells.Results Compared with group normal 0μmol·L^(-1),the expression of NF-kB,IL-1βand TNF-αin group normal 800μmol·L^(-1) was significantly decreased(P all<0.01);Compared with group tumor 0μmol·L^(-1),the expression of NF-kB,IL-1βand TNF-αin group tumor 400 and 800μmol·L^(-1) were significantly decreased(P all<0.01)and the cell proliferation,invasion and migration ability of group tumor 200,400 and 800μmol·L^(-1) were significantly decreased(P all<0.01).Conclusion EPG can significantly down-regulate the expression of inflammatory factors and inhibit the malignant behavior of osteosarcoma cells.