香榧纯露的挥发性成分及抗氧化活性研究

Volatile Composition and Antioxidant Activity of Torreya grandis Hydrosols

为了研究香榧纯露的稳定性及体内外抗氧化活性,以香榧假种皮水蒸气提取精油的副产物香榧纯露为试验材料,采用LUMi Sizer稳定性分析仪对香榧纯露的稳定性进行了评价,并通过气相色谱-质谱分析(GC-MS)技术对纯露的挥发性成分进行分析。在此基础之上,通过体外自由基清除实验评估了香榧纯露的体外抗氧化能力,并利用秀丽隐杆线虫模型对香榧纯露体内抗氧化活性进行了评价。结果表明:经膜过滤处理的香榧纯露不稳定系数接近于0(0.007±0.003),具有更好的稳定性。采用GC-MS技术共鉴定出49种挥发性成分,以醇类化合物(41.82%)为主,共21种;其次为萜类化合物(5.27%),共7种;其他还包括酚类化合物(5种)、烃类化合物(5种)、醛类化合物(3种)及酯类化合物等。体外抗氧化活性结果表明,香榧纯露对DPPH、ABTS自由基清除能力与浓度呈正相关,最高浓度下清除率分别为67.3%±1.2%和84.2%±3.7%。此外,香榧纯露可显著提高秀丽隐杆线虫抗氧化应激性能及良好的延长寿命的作用(P<0.05),有望开发为抗氧化、抗衰老功效的新型食品或化妆品原料。

The purpose of this experiment was to study the stability and antioxidant activity of Torreya grandis hydrosols in vivo and in vitro.The work aims to study the stability,volatile composition and antioxidant activity of Torreya grandis hydrosols.The stability of untreated and filter-treated hydrosols was evaluated by LUMi Sizer stability analyzer,and the volatile components of hydrosols were analyzed by gas chromatography-mass spectrometry(GC-MS).In addition,the antioxidant activity in vitro and in vivo was evaluated by free radical scavenging experiments and C.elegans tests respectively.The results showed that the filter-treated hydrosols revealed better stability as compared to untreated hydrosols,with the instability coefficient close to 0(0.007±0.003).GC-MS demonstrated that a total of 49 volatile components were identified for the hydrosols,including 21 alcohols(41.82%),7 terpenoids(5.27%),5 phenols,5 hydrocarbons,3 aldehydes and some other types of volatile compounds.DPPH and ABTS free radical scavenging experiments demonstrated that T.grandis hydrosols revealed good antioxidant activity with radical scavenging rate of67.3%±1.2% and 84.2%±3.7%,respectively.Furthermore,the hydrosols revealed significant antioxidant performance in C.elegans and prolong the life span of C.elegans(P<0.05).These results may help in developing new antioxidant and antiaging ingredients for food and cosmetic products use.