微波法制备猕猴桃生物质碳点及应用于金银花中Fe^(3+)的检测

Preparation of Kiwifruit Biomass Carbon Dots by Microwave Method and Application of Fe^(3+) Detection in Honeysuckle

为建立一种快速检测Fe^(3+)的方法,以猕猴桃汁为碳源,乙二胺为表面修饰剂,采用微波法制备猕猴桃生物质碳点(CDs)。制备的生物质碳点富含-OH、-NH和C-O等基团,粒径约为2.5 nm,易溶于水且稳定。该碳点在365 nm紫外光照射下发出蓝色荧光。基于Fe^(3+)对猕猴桃生物质碳点荧光猝灭效应,进而建立一种检测Fe^(3+)的方法。当Fe^(3+)的加入量在0.2~10μmol/L范围内,碳点的荧光猝灭程度(F/F_(0))随Fe^(3+)浓度的增加而呈线性下降(R^(2)=0.9934),检出限(S/N=3)为0.12μmol/L。将该方法用于测定金银花中Fe^(3+)的含量,其回收率在98.82%~103.20%,RSD介于1.67%~2.05%之间。该检测方法操作简便,快速,选择性好。

In order to establish a rapid detection method for Fe^(3+),the kiwifruit biomass carbon dots(CDs)were prepared by microwave method with kiwifruit juice as carbon source and ethylenediamine as surface modifier.The CDs were rich in-OH,-NH and C-O groups,had an average particle size about 2.5 nm,which were soluble in water and stable.The CDs emitted blue fluorescence under 365 nm ultraviolet light.A new and rapid detection method for Fe^(3+)was established with the CDs based on the fluorescence quenching effect of Fe^(3+).When the addition amount of Fe^(3+)from 0.2 to 10μmol/L,the fluorescent intensity(F/F_(0))of CDs decreased linearly with the increase of Fe^(3+)(R^(2)=0.9934),and the limit of detection(S/N=3)was 0.12μmol/L.This detection method was applied to the determination of Fe^(3+)content and the recovery was 98.82%~103.20%,RSD was between 1.67% and 2.05%.The operation of this detection method was simple,rapid and strong selective.