地参游离酚对CCl_(4)所致肝细胞损伤细胞凋亡、细胞周期及炎症因子的影响

Effects of Free Phenolics from Lycopus lucidus Turcz.on Apoptosis,Cell Cycle and Inflammatory Factors in CCl_(4) Induced Hepatocyte Injury

通过体外实验,研究地参游离酚对CCl_(4)所致肝细胞损伤细胞凋亡、细胞周期及炎症因子的影响。采用流式细胞术分析细胞凋亡及细胞周期的分布,采用试剂盒测定肿瘤坏死因子α(TNF-α)、白介素8(IL-8)的含量和天冬氨酸半胱氨酸蛋白酶3(Caspase-3)活化程度,同时采用免疫组化检测环氧化酶-2(COX-2)、白介素6(IL-6)和诱导型一氧化氮合酶(iNOS)的蛋白表达水平。结果表明,与模型组相比,地参游离酚能显著降低CCl_(4)引起的细胞凋亡,影响细胞周期的分布,能显著降低炎症因子TNF-α、IL-8的含量并阻滞Caspase-3的活化程度(P<0.05),并不同程度降低COX-2、IL-6和iNOS蛋白表达水平(P<0.05)。地参游离酚通过抑制细胞凋亡、影响细胞周期分布和抑制炎症反应保护CCl_(4)所致的肝细胞损伤。

To investigate the effects of free phenolics from Lycopus lucidus Turcz.on apoptosis,cell cycle and inflammatory factors in CCl_(4)induced hepatocyte injury by using a in vitro experiment.Apoptosis and the distribution of cell cycle was analyzed by flow cytometry.The contents of tumor necrosis factor-α(TNF-α),interleukin-8(IL-8)and the activation of Caspase-3 were measured by kits.The protein expression levels of cyclooxygenase-2(COX-2),inducible nitric oxide synthase(iNOS)and interleukin-6(IL-6)were also detected by immunohistochemistry.The results showed that compared with the model group,the free phenolics from Lycopus lucidus Turcz.could significantly reduce the apoptosis induced by CCl_(4)and affect the distribution of cell cycle.In addition,the free phenolics from Lycopus lucidus Turcz.decreased the contents of TNF-α and IL-8 and blocked the activation of Caspase-3(P<0.05).The free phenolics from Lycopus lucidus Turcz.also decreased the expression of COX-2,IL-6 and iNOS(P<0.05).The free phenolics from Lycopus lucidus Turcz.could protect the CCl_(4)induced hepatocyte injury through inhibiting apoptosis,affecting cell cycle distribution and inhibiting inflammatory reaction.