衰老对2种猕猴血液基因表达的影响及与人类的对比

Effect of Ageing on the Blood Gene Expression of Two Macaques and the Comparison with Humans

衰老是导致免疫功能和代谢活动下降的危险因素。作为最常用的非人灵长类动物模型,猕猴属Macaca被用来探索与衰老过程相关的因素。为了比较2种猕猴和人类在衰老过程基因表达变化的异同,本研究收集比较了3个物种的血液转录组。在藏酋猴M.thibetana(TM)、普通猕猴M.mulatta(CR)和人类中分别获得了2523个、2138个和1497个与年龄相关的差异表达基因(DEGs);三者共同上调的23个,下调的38个。共同下调的38个DEGs富集到了B细胞受体信号通路(ko04662),体液免疫上,TMs最强,CRs最弱。免疫功能是3个物种在衰老过程中共同的功能改变,但改变程度不同。基因表达模式的相似性和差异性的发现将为猕猴进化和生物医学研究提供基础,同时为将猕猴衰老过程基因表达变化的结果推广到人类衰老相关研究提供参考。

Aging is a risk factor leading to the decline of immune function and metabolic activity.As the most commonly used non-human primate model,macaques are used to explore factors related to aging process.In this study,in order to compare the similarities and differences between the aging process of macaque and human,and to extend the results of macaque research to human,we collected and compared the blood transcriptomes of two kinds of macaques and human.The results showed that there were 2523,2138 and 1497 age-related differentially expressed genes(DEGs)in Macaca thibetana(TMs),M.mulatta(CRs)and human,respectively.Specifically,38 down-regulated DEGs enriched in the B cell receptor signaling pathway(ko04662)and 23 up-regulated DEGs with no significant enrichment were detected in all the three species.However,based on the prediction of KEGG pathway,TMs might have the strongest humoral immunity,followed by human and CRs.In conclusion,the similarities and differences between the aging processes of macaques and human identified in this study provides a basis for further studying the evolution of macaque and human-related biomedical research.