EGFR和CD44蛋白在结直肠腺癌中的表达及其临床病理意义

Expression of EGFR and CD44 in colorectal adenocarcinoma and its clinicopathological significance

目的 检测表皮生长因子受体(EGFR)和CD44在结直肠腺癌组织中的表达,探讨两者与结直肠腺癌的临床病理关系及两者之间的相关性。方法 应用免疫组化SP法检测150例结直肠腺癌组织中EGFR和CD44的表达。结果EGFR和CD44在结直肠腺癌中的表达率分别为40.67%和51.33%;EGFR阳性表达与肿瘤大小和淋巴结转移有关(P <0.05),而与患者性别、年龄、肿瘤部位、分化程度、浸润深度无关联(P>0.05)。CD44的阳性表达与肿瘤浸润深度、淋巴结转移有明显关联(P <0.05),而与患者性别、年龄、肿瘤部位、肿瘤大小、分化程度无关联(P>0.05)。EGFR和CD44的表达呈弱相关性(C=0.279,P <0.001)。结论 EGFR和CD44蛋白的阳性表达与肿瘤的淋巴结转移和浸润等预后相关因素有关联,可能成为肿瘤预后判断的依据。两者蛋白表达呈弱相关,提示两者之间可能存在协同作用。联合检测EGFR和CD44可有助于判断结直肠腺癌局部侵袭和远处转移的能力。

Objective To detect the expression of epidermal growth factor receptor(EGFR)and CD44 in colorectal adenocarcinoma,and to explore their association with clinicopathology of colorectal adenocarcinoma as well as the correlation between EGFR and CD44.Methods The immunohistochemical SP method was used to detect the expression of EGFR and CD44 in 150 cases of colorectal adenocarcinoma.Results The expression rates of EGFR and CD44 in colorectal adenocarcinoma were 40.67%and 51.33%,respectively.The positive expression of EGFR was related with tumor size and lymph node metastasis(P<0.05),but not related with gender,age,tumor location,tumor differentiation and the depth of tumor invasion(P>0.05).The positive expression of CD44 was significantly related with the depth of tumor invasion and lymph node metastasis(P<0.05),but not with gender,age,tumor location,tumor size and tumor differentiation(P>0.05).The expression of EGFR was weakly correlated with that of CD44(C=0.279,P<0.001).Conclusion The positive expression of EGFR and CD44 protein is associated with prognostic factors including lymph node metastasis and invasion of tumors,which may be the basis for tumor prognosis.The expressions of these two proteins are weakly correlated,suggesting that there might be synergistic effect between these two proteins.Combined detection of EGFR and CD44 may be helpful for judging local invasion and distant metastasis of colorectal adenocarcinoma.