中等强度运动训练结合仙灵骨葆胶囊对去势大鼠骨代谢的影响

Effects of Moderate Intensity Exercise Training Combined with Xianlinggubao Capsule on Bone Metabolism in Ovariectomized Rats

目的观察中强度运动训练结合仙灵骨葆胶囊对去卵巢大鼠骨密度(bone mineral density,BMD)、骨代谢及股骨生物力学的影响,为防治骨质疏松提供实验室参考。方法50只雌性SD大鼠随机分为5组,每组10只。A组为正常对照,B组切除卵巢1周后灌胃给予1 mL生理盐水,C组切除卵巢1周后进行中等强度的运动训练(运动速度为20 m/min,每天持续时间为60 min,连续5 d/周),D组切除卵巢1周后灌胃给予1 mL仙灵骨葆胶囊[0.4 g/(kg·d)],E组切除卵巢1周后灌胃给予1 mL仙灵骨葆胶囊+中等强度的运动训练,连续治疗8周后,进行血液生化指标检测,以及股骨、L5椎体BMD、Micro-CT检测与生物力学检测。结果与B组比较,C~E组的血液生化指标均有不同程度的改善,L5椎体、股骨骨密度升高,股骨(或L5椎体)的骨体积分数、骨小梁数目、骨小梁厚度升高,骨小梁间隙与结构模型指数降低,L5椎体最大载荷、最大挠度及最大应力升高,股骨最大载荷、弹性载荷、弹性挠度、弹性模量、弹性应力、最大应力及弹性挠度升高,其中均以E组效果最明显。结论中等强度运动训练结合仙灵骨葆胶囊治疗可提升去卵巢大鼠BMD,改善骨代谢与骨微结构,提高骨力学性能,两者具有协同作用。

Objective To observe the effects of moderate intensity exercise training combined with Xianlinggubao capsule on bone mineral density(BMD),bone metabolism and femoral biomechanics of ovariectomized rats,so as to provide lab references for osteoporosis prevention.Methods Fifty female SD rats were randomly divided into 5 groups,10 rats in each group.Group A was the normal control;Group B was given 1 mL normal saline by gavage after ovariectomy for one week;Group C was given moderate intensity exercise training(exercise speed was 20 m/min,lasting for 60 min per day,continuous 5 days per week);Group D was given 1 mL Xianlinggubao capsule[0.4 g/(kg·d)]after 1-week ovariectomy;Group E was was given both 1 mL Xianlinggubao capsule and moderate intensity exercise training after 1-week ovariectomy.After 8 weeks of continuous treatment,blood biochemical indexes,BMD,micro CT and biomechanics of the femur and L5 vertebral body were detected.Results Compared with group B,the blood biochemical indexes of Group C-E were improved in varying degrees,the BMD of L5 vertebral body and femur were increased,the bone volume fraction,trabecular number and trabecular thickness of femur(or L5 vertebral body)were increased,the trabecular space and structural model index were decreased,the maximum load,maximum deflection and maximum stress of L5 vertebral body were increased,and the maximum stress of femur was increased.The maximum load,elastic load,elastic deflection,elastic modulus,elastic stress,maximum stress and elastic deflection increased,and the effect of Group E was the most obvious.Conclusions Moderate intensity exercise training combined with Xianlinggubao capsule can improve BMD,bone metabolism and bone microstructure,and improve bone mechanical properties of ovariectomized rats.