银杏内酯B通过MMP9/STAT3信号通路对肺癌细胞A549增殖、迁移、侵袭和凋亡的影响

Effects of Ginkgolide B on Proliferation,Migration,Invasion and Apoptosis of Lung Cancer Cell A549 through MMP9/STAT3 Signaling Pathway

目的:探究银杏内酯B(ginkgolide B,GB)对肺癌细胞A549恶性生物学行为的影响。方法:将A549细胞随机分为对照组、银杏内酯B低剂量组、银杏内酯B中剂量组和银杏内酯B高剂量组,除对照组外,分别采用25、50、100μmol/L剂量的银杏内酯B处理银杏内酯B低、中、高剂量组的A549细胞。MTT法检测细胞增殖;Transwell法检测细胞侵袭;划痕法检测细胞迁移;流式细胞仪检测细胞凋亡率;Western Blot法检测Ki67、增殖细胞核抗原(PCNA)、Bax、Bcl-2、Caspase-3、基质金属蛋白酶-9(MMP-9)、信号转导和转录激活因子3(STAT3)和p-STAT3蛋白表达水平。结果:与对照组相比较,银杏内酯B中、高剂量组细胞活力降低,Ki67、PCNA蛋白水平显著降低,侵袭细胞数降低,划痕愈合率降低,细胞凋亡率升高,Bax/Bcl-2、Cleaved Caspase/Caspase-3比值升高,MMP-9蛋白表达水平和p-STAT3/STAT3比值降低,差异均具有统计学意义(P<0.05)。结论:银杏内酯B可通过调控MMP9/STAT3通路抑制肺癌细胞A549恶性生物学行为。

Objective:To investigate the effect of ginkgolide B(GB)on malignant biological behavior of lung cancer cell A549.Methods:A549 cells were randomly divided into the control group and the GB groups of low-dose,medium-dose and high-dose(25μmol/L,50μmol/L and 100μmol/L).MTT assay was used to detect cell proliferation.Cell invasion was detected by Transwell.Cell migration was detected by scratch method.Cell apoptosis rate was detected by flow cytometry.Western Blot was used to detect the protein levels of Ki67,PCNA,Bax,Bcl-2,Caspase-3,MMP-9,STAT3 and p-STAT3.Results:Compared to those in the control group,the cell viability was decreased,the protein levels of Ki67 and PCNA were significantly decreased,the number of invasive cells was decreased,scratch healing rate was decreased,cell apoptosis rate was increased,the ratios of Bax/Bcl-2 and Cleaved Caspase/Caspase-3 were increased,the protein expression of MMP-9 and the ratio of p-STAT3/STAT3 were decreased in the GB groups of medium-dose and high-dose(P<0.05).Conclusion:GB can inhibit the malignant biological behavior of A549 lung cancer cells by regulating MMP9/STAT3 pathway.