lncRNA PVT1靶向miR-146a对胃癌MKN-45细胞增殖和侵袭的影响

Effects of lnc RNA PVT1 by targeting miR-146a on the proliferation and invasion of gastric cancer MKN-45 cells

目的探讨lncRNA RNA PVT1对胃癌MKN-45细胞增殖和侵袭的影响及可能机制。方法将PVT1抑制物转染胃癌MKN-45细胞,将细胞分为对照组(untreated组)、阴性对照组(si-NC组)及沉默PVT1组(si-PVT1组),实时定量PCR方法检测各组MKN-45细胞PVT1和miR-146a的表达,双荧光素酶报告基因实验分析PVT1和miR-146a的靶向关系,MTT法和Edu染色法检测各组MKN-45细胞的增殖能力,Transwell实验检测各组MKN-45细胞的侵袭能力。结果转染PVT1抑制物能够使MKN-45细胞PVT1的表达下降,miR-146a的表达升高(P<0.05),双荧光素酶实验表明PVT1可以靶向调控miR-146a。MTT、EdU和Transwell实验结果显示,抑制PVT1表达后,MKN-45细胞的增殖和侵袭能力下降(P<0.05)。结论沉默lncRNA PVT1使胃癌细胞MKN-45的增殖和侵袭能力下降,其机制可能与负调控miR-146a表达有关。

Objective To investigate the effect and mechanism of long-chain non coding RNA PVT1 on the proliferation and invasion of gastric cancer MKN-45 cells.Methods The PVT1 inhibitor was transfected into MKN-45 cells,the cells were divided into untreated group(control group),si-NC group(negative control group)and si-PVT1 group.The expression of lncRNA PVT1 and miR-146 a was detected by quantitative real-time PCR,and the target relationship between lncRNA PVT1 and miR-146 a was verified by double luciferase assay.The proliferation ability of MKN-45 cells was detected by MTT and EdU,the invasion ability was detected by Transwell.Results After silencing lncRNA PVT1,the expression of lncRNA PVT1 was significantly decreased and the expression of miR-146 a was up-regulated.Doubleluciferase assay confirmed that PVT1 can regulate the expression of miR-146 a.The inhibition of lncRNA PVT1 expression significantly reduced the proliferation and invasion of MKN-45 cells.Conclusion lncRNA PVT1 may regulate the proliferation and invasion of MKN-45 cells via miR-146 a pathway.