婴儿配方奶粉中双酚A类化合物成分分析标准物质候选物研制

Development of reference material candidates for bisphenol A analogues in infant formula milk powders

目的通过研制婴儿配方奶粉中双酚A、双酚S、双酚F和双酚AF成分分析标准物质候选物,为后续进一步形成基体标准物质提供技术基础和方法学参考,填补该方面的研究空白。方法选择市售婴儿配方奶粉为样品基质,采用加水溶解、标准添加、均质、干燥、粉碎、筛分和分装等工序制备标准物质候选物,以同位素稀释-液相色谱-串联质谱法进行目标物含量测定。随机抽取25瓶样品进行均匀性检验,采用F检验进行数据的统计分析。在室温25℃下放置0、1、3、6和12个月、60℃下放置0、1、3、5和7 d后分别对候选物样品进行测定,通过一元线性模拟方程评估其长期和短期稳定性。结果研制的基体标准物质候选物均匀性良好,特征值在考察的时间范围内无明显变化,符合标准物质的预期要求。结论本研究率先开展了食品中双酚A类化合物成分分析标准物质的研制工作,有望为国内食品中双酚A类化合物监测技术的提升提供技术支持和质控参考。

Objective Reference material candidates of bisphenol A, bisphenol S, bisphenol F and bisphenol AF in infant formula milk powders were prepared, in order to provide technical basis and methodology for the further formation of certified reference materials, and fill the research gap in this field. Methods The commercial infant formula milk powders were selected to prepare the reference material candidates by the processes of water dissolution, standard addition, homogenization, drying, crushing, screening and packaging. The concentrations of the target compounds were determined by isotope dilution-liquid chromatography tandem mass spectrometry. 25 bottles of samples were randomly selected for homogeneity study, and F test was used for statistical analysis. The long-term and short-term stability were studied by the quantitative analysis of samples stored at 25 ℃ for 12 months and at 60 ℃ for 7 d, respectively, and evaluated by univariate linear simulation equation. Results The homogeneity and stability result of the prepared reference material candidates were satisfactory, which met the expected requirements. Conclusion This work was expected to fill the gap in preparation of matrix reference materials for bisphenol A analogues, and provid material basis and technical support for the monitoring of bisphenol A analogues in food.