碱性成纤维细胞生长因子对激光烧伤大兔皮肤愈合的影响分析

Analysis of the Effect of Basic Fibroblast Growth Factor on the Skin Healing of Laser Burned Rabbits

目的:探讨碱性成纤维细胞生长因子对激光烧伤大兔皮肤愈合的影响分析。方法:通过热辐射仪激光灼烧对大兔耳朵进行烧伤处理,根据实验需求,将其随机分为三组:对照组,bFGF组,bFGF+DAPT组。通过ImageJ软件测量伤口面积和疤痕组织的厚度,并定期计算残余伤口面积率和疤痕指数。通过组织学分析大兔伤口愈合的新血管生成量。通过蛋白印迹分析Notch1、Jagged1和Hes1的蛋白表达。通过免疫荧光分析愈合后的皮肤中α-SMA,Col I和Col III的相对蛋白水平。结果:bFGF组较对照组的疤痕指数降低(P<0.05),bFGF+DAPT组较bFGF组疤痕指数升高(P<0.05)。bFGF组较对照组的愈合面积增加(P<0.05),bFGF+DAPT组较bFGF组愈合面积降低(P<0.05)。与对照组相比,bFGF组的愈合时间明显缩短,较低的残余伤口面积和较低的疤痕指数(P<0.05),而bFGF+DAPT组表现出明显的愈合延迟和较高的疤痕指数(P<0.05)。bFGF组较对照组的新血管生成量增加(P<0.05),bFGF+DAPT组较bFGF组心血管生成量减少(P<0.05)。bFGF组较对照组的肉芽组织平均厚度增加,表皮间隙的闭合百分比升高(P<0.05),bFGF+DAPT组较bFGF组肉芽组织平均厚度增加、表皮间隙的闭合百分比减少(P<0.05)。H&E染色进行组织学分析发现,与对照组和bFGF+DAPT组相比,bFGF组出现明显的再上皮化和新血管形成,愈合效率较高(P<0.05)。bFGF组较对照组Notch1、Jagged1和Hes1的蛋白表达升高(P<0.05),bFGF+DAPT组较bFGF组Notch1、Jagged1和Hes1的蛋白表达降低(P<0.05)。bFGF组较对照组α-SMA,Col I和Col III的蛋白表达降低(P<0.05),bFGF+DAPT组较bFGF组表达升高(P<0.05)。结论:bFGF可以通过促进ESC的增殖并通过激活Notch1/Jagged1途径抑制其向肌成纤维细胞(Myofibroblasts,MFB)的分化加快伤口愈合,减少疤痕形成。

Objective:To explore the effect of basic fibroblast growth factor on the skin healing of laser burned rabbits.Methods:The rabbit ears were burned by laser burning with a thermal radiometer.According to the needs of the experiment,they were randomly divided into three groups:control group,bFGF group,bFGF+DAPT group.The wound area and scar tissue thickness are measured by ImageJ software,and the residual wound area rate and scar index are calculated regularly.Histological analysis of the amount of new angiogenesis in the wound healing of rabbits.The protein expression of Notch1,Jagged1 and Hes1 was analyzed by Western blot.The relative protein levels ofα-SMA,Col I and Col III in the healed skin were analyzed by immunofluorescence.Results:The scar index of the b FGF group was lower than that of the control group(P<0.05),and the scar index of the bFGF+DAPT group was higher than that of the bFGF group(P<0.05).Compared with the control group,the healing area of the bFGF group was increased(P<0.05),and the healing area of the b FGF+DAPT group was lower than that of the bFGF group(P<0.05).Compared with the control group,the healing time of the bFGF group was significantly shorter,with a lower residual wound area and a lower scar index(P<0.05),while the bFGF+DAPT group showed obvious delayed healing and a higher scar index(P<0.05).The amount of neovascularization in the bFGF group was higher than that in the control group(P<0.05),and the amount of cardiovascular production in the bFGF+DAPT group was lower than that in the b FGF group(P<0.05).Compared with the control group,the average thickness of granulation tissue in the bFGF group increased,and the closure percentage of epidermal space increased(P<0.05).Compared with the bFGF group,the average thickness of granulation tissue in the b FGF+DAPT group increased,and the closure percentage of epidermal space decreased(P<0.05).H&E staining for histological analysis showed that compared with the control group and bFGF+DAPT group,the bFGF group had obvious re-epithelialization and new blood vessel formation,and the healing efficiency was higher(P<0.05).The protein expression of Notch1,Jagged1 and Hes1 in the b FGF group was higher than that in the control group(P<0.05),and the protein expression of Notch1,Jagged1 and Hes1 in the bFGF+DAPT group was lower than that in the bFGF group(P<0.05).The protein expression ofα-SMA,Col I and Col III in the bFGF group was lower than that in the control group(P<0.05),and the expression in the bFGF+DAPT group was higher than that in the bFGF group(P<0.05).Conclusion:bFGF can promote the proliferation of ESC and inhibit its differentiation into myofibroblasts(MFB)by activating the Notch1/Jagged1 pathway to accelerate wound healing and reduce scar formation.