兔前交叉韧带损伤后膝关节本体感觉、残端血运及膝关节腔尿激酶型纤溶酶原激活物变化的实验研究

Experimental Study on Changes of Knee Proprioception, Stump Blood Supply and Urokinase-Type Plasminogen Activator in the Knee Cavity after Anterior Cruciate Ligament Injury in Rabbit

目的:研究兔前交叉韧带(ACL)损伤后膝关节本体感觉、残端血运及膝关节腔尿激酶型纤溶酶原激活物(u-PA)的变化情况。方法:选取80只新西兰兔进行研究,将其以随机数字表法分作模型组及空白对照组各40只。模型组建立单侧ACL损伤模型,空白对照组仅切开关节。比较两组术前及术后2周、4周、8周时膝关节本体感觉、残端血运及膝关节腔u-PA水平的差异。结果:模型组新西兰兔术后2周、4周、8周时的体感诱发电位(SEP)、肌电图(EMG)潜伏期均高于空白对照组,而SEP、EMG波幅均低于空白对照组(P<0.05)。模型组新西兰兔术后2周、4周、8周时的残端组织微血管密度分别为(2.04±0.24)n/mm2、(2.75±0.61)n/mm2、(1.60±0.33)n/mm2,均高于空白对照组的(1.34±0.24)n/mm2、(1.34±0.25)n/mm2、(1.35±0.26)n/mm2,差异均有统计学意义(P<0.05)。模型组新西兰兔术后2周、4周、8周时的膝关节液u-PA水平分别为(173.97±14.29)pg/mL、(188.37±15.82)pg/mL、(171.38±14.76)pg/mL,均高于空白对照组的(158.02±10.18)pg/mL、(157.68±10.20)pg/mL、(157.37±10.07)pg/mL,差异均有统计学意义(P<0.05)。结论:ACL损伤后会在不同程度上影响膝关节本体感觉、残端血运及膝关节腔u-PA含量,值得临床进一步研究。

Objective:To study the changes of knee proprioception,stump blood supply and urokinase-type plasminogen activator(u-PA)in the knee cavity after anterior cruciate ligament(ACL)injury in rabbits.Methods:80 New Zealand rabbits were selected for the study,and they were divided into model group and blank control group with 40 rabbits each by random number table method.A unilateral ACL injury model was established in the model group,and only the joint was cut open in the blank control group.The knee proprioception,stump blood supply and u-PA level in the knee cavity were compared between the two groups before and 2,4 and 8 weeks after operation.Results:The incubation period of somatosensory evoked potential(SEP)and electromyography(EMG)in the model group were higher than those in the blank control group at 2,4 and 8 weeks after operation,while the amplitude of SEP and EMG were lower than those in the blank control group(P<0.05).The microvessel density of stump tissue in the model group at 2,4 and 8 weeks after operation were(2.04±0.24)n/mm2,(2.75±0.61)n/mm2 and(1.60±0.33)n/mm2 respectively,which were higher than those in the blank control group(1.34±0.24)n/mm2,(1.34±0.25)n/mm2,(1.35±0.26)n/mm2,the differences were statistically significant(P<0.05).The u-PA level of articular fluid in the model group at 2,4 and 8 weeks after operation were(173.97±14.29)pg/mL,(188.37±15.82)pg/mL,(168.38±14.76)pg/mL respectively,which were higher than those in blank control group(158.02±10.18)pg/mL,(157.68±10.20)pg/m L,(157.37±10.07)pg/mL,the differences were statistically significant(P<0.05).Conclusion:After ACL injury,the knee proprioception,stump blood supply and u-PA content in the knee cavity can be affected to different degrees,which is worthy of further clinical study.