紫娟茶中甲基化儿茶素提取工艺优化

Study on extraction process of methylated catechin from Zijuan tea by response surface methodology

目的:优化紫娟茶中甲基化儿茶素的提取工艺。方法:以酸性乙醇为溶剂,考察提取时间、提取温度和液料比对紫娟茶中甲基化儿茶素提取率的影响。结果:紫娟茶中甲基化儿茶素的最佳提取条件为提取时间100 min,提取温度75℃,料液比(m_(紫娟茶)∶V_(溶剂))1∶74(g/mL),此条件下甲基化儿茶素质量浓度为26.7 mg/g,与验证值的相对误差为1.48%。结论:运用HPLC分析和响应面分析法优化紫娟茶中甲基化儿茶素的提取工艺简单、可行。

Objective:optimizing the extraction process of methylated catechin from Zijuan tea.Methods:Using acid ethanol as extraction solvent,based on three single factor experiments,the extraction time,extraction temperature and liquid-solid ratio were used to investigate the interaction of the above three factors on the extraction rate of methylated catechin from Zijuan tea.Results:The optimum extraction conditions were as follows:extraction time 100 min,extraction temperature 75℃,solid-liquid ratio(m_(Zijuan tea)∶V_(Solvent))1∶74(g/mL);under these conditions,the mass concentration of methylated catechin in the extract could reach 26.7 mg/g,and the relative error with the verification value was 1.48%.Conclusion:HPLC and response surface methods were used to optimize the extraction process of methylated catechins in Zijuan tea.The process is simple and the verification results are reasonable.