隐绿原酸对H9N2-AIV致小鼠肺损伤的免疫调节和抗氧化效果

Effect of cryptochlorogenic acid on immunomodulatory and antioxidant in mice of lung injury induced by H9N2-AIV

为探讨隐绿原酸(4-CQA)对感染H9N2亚型禽流感(H9N2-AIV)病毒的小鼠造成肺损伤的修复作用,将90只6~8周龄健康昆明系小鼠随机分为病毒(H9N2)感染组、H9N2+4-CQA组、空白对照组、4-CQA对照组,用H9N2-AIV滴鼻复制小鼠肺损伤模型并灌胃4-CQA干预。观察各组小鼠肺组织病理学变化;测定肺指数及肺内丙二醛(MDA)、总超氧化物歧化酶(T-SOD)、髓过氧化物酶(MPO)含量;测定脾内T淋巴细胞亚群CD4^(+)、CD8^(+)百分比及比率(CD4^(+)/CD8^(+))。结果显示,H9N2组小鼠表现出精神沉郁、呼吸急促;肺指数上升、脾指数下降;肺组织病理学表现为肺泡萎缩、肺泡隔增厚、局部肺实变;T淋巴细胞亚群CD4^(+)/CD8^(+)比率显著下降(P<0.05);肺内T-SOD活性显著下降、MDA含量显著增加、MPO活性显著升高(P<0.05)。4-CQA干预后,小鼠肺指数下降、脾指数上升;肺组织病理学损伤减轻;T淋巴细胞亚群CD4^(+)/CD8^(+)比率显著上升(P<0.05);肺内T-SOD活性显著升高,MDA含量显著降低,MPO活性显著下降(P<0.05)。得出4-CQA可通过维持机体免疫平衡、降低机体氧化损伤来缓解由H9N2亚型AIV诱导的小鼠肺损伤。

To investigate the effect of cryptochlorogenic acid(4-CQA)on lung injury and recovery in mice infected with avian influenza H9N2 virus,ninety SPF KM mice(6-8 weeks old)were randomly divided into H9N2 group,H9N2+4-CQA group,control group and 4-CQA group.Then H9N2-AIV was used to induce lung injury and received 4-CQA intragastric administration respectively in mice.The lung histopathlogy changes,the lung index and spleen index,and the contents of malondialdehyde(MDA),total superoxide dismutase(T-SOD)and myeloperoxidase(MPO)in the lung tissue homogenate,and the ratio of T cell subgroups(CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+))were observed.The results showed that the group of H9N2 presented depression and shortness of breath;lung index increased and spleen index decreased;histopathological manifestations of lung were alveolar atrophy,alveolar septal thickening and local lung consolidation;CD4^(+)/CD8^(+)ratio of T lymphocyte subsets decreased significantly(P<0.05),activity of T-SOD decreased significantly,content of MDA increased significantly,and activity of MPO increased significantly(P<0.05).After 4-CQA treatment,lung index decreased and spleen index increased;lung histopathological damage was alleviated;CD4^(+)/CD8^(+)ratio of T cell subgroups was significantly increased(P<0.05);T-SOD and MPO activity in lung were significantly increased and the content of MDA was significantly decreased(P<0.05).It was concluded that 4-CQA can alleviate the lung injury induced by avian influenza H9N2 virus by maintaining immune balance and reducing oxidative damage.