莪术醇对肝细胞癌miR-629、miR-24、miR-130a、miR-17的影响

Effects of Curcumol on miR-629,miR-24,miR-130a and miR-17 in Hepatocellular Carcinoma

目的探讨莪术醇对肝癌miR-629、miR-24、miR-130a和miR-17表达的影响,从microRNAs角度揭示莪术醇抗肝癌的机制。方法取人肝癌细胞株HepG2进行培养,待细胞生长良好,将其分为对照组、50μg/ml莪术醇及100μg/ml莪术醇药物组,每组3瓶。莪术醇(50、100μg/ml,少量DMSO溶解)药物组换入含莪术醇的培养液作用,对照组予少量DMSO,作用于人肝癌细胞株HepG2。48 h后提取各组总RNA,实时荧光定量PCR检测莪术醇对miR-17、miR-130和miR-24、miR-629表达的调控作用。结果两个莪术醇药物组中miR-130a-5P、miR-17-3P的表达水平均高于对照组,差异有统计学意义(P<0.05);两个莪术醇药物组中miR-629-3P、miR-24-1-3P表达水平均低于对照组,差异有统计学意义(P<0.05);50μg/ml莪术醇药物组的miR-17-3P表达水平高于100μg/ml莪术醇药物组,差异有统计学意义(P<0.05);两个莪术醇药物组其余miRNAs表达水平比较,差异无统计学意义(P>0.05)。结论莪术醇可能通过上调miR-130a-5P、miR-17-3P,下调miR-629-3P、miR-24-1-3P的表达发挥抗肝癌作用。

Objective To investigate the effects of curcumol on the expression of miR-629,miR-24,miR-130a and miR-17 in liver cancer,and to reveal the mechanism of curcumol against liver cancer from the perspective of microRNAs.Methods Human hepatocellular carcinoma cell line HepG2 was cultured.When the cells grew well,they were divided into control group,50μg/ml curcumol group and 100μg/ml curcumol group,with three bottles in each group.The curcumol(50 and 100μg/ml,dissolved in a small amount of DMSO)drug group was replaced with the culture medium containing curcumol,and the control group was treated with a small amount of DMSO,acting on human liver cancer cell line HepG2.After 48 h,total RNA was extracted from each group,and the regulation of curcumol on the expression of miR-17,miR-130,miR-24 and miR-629 was detected by real-time quantitative PCR.Results The expression levels of miR-130a-5P and miR-17-3P in the two curcumol groups were higher than those in the control group,and the difference was statistically significant(P<0.05).The expression levels of miR-629-3P and miR-24-1-3P in the two curcumol groups were lower than those in the control group,and the difference was statistically significant(P<0.05).The expression level of miR-17-3P in the 50μg/ml curcumol group was higher than that in the 100μg/ml curcumol group,and the difference was statistically significant(P<0.05).There was no significant difference in the expression level of miRNAs between the two curcumol groups(P>0.05).Conclusion Curcumol may play an anti-HCC role by up-regulating miR-130a-5p and miR-17-3p and down-regulating the expression of miR-629-3p and miR-24-1-3p.