响应面优化藜麦蛋白提取及对力竭运动大鼠血流动力学和氧化应激的影响

Response surface optimization of quinoa protein extraction and its effect on hemodynamics and oxidative stress in exhausted exercise rats

采用响应面优化酶解法提取藜麦蛋白,并研究其对力竭运动大鼠血流动力学和氧化应激的影响。藜麦蛋白的最佳提取工艺方案:液料比21∶1(mL/g)、提取温度52℃、酶添加量4.2%(以藜麦粉末质量为基准添加)。在此条件下,藜麦蛋白平均提取率为71.26%,与理论预测值相差不大。大鼠力竭运动试验表明,藜麦蛋白均可以提高大鼠的左心室收缩压值(LVSP)和降低左心室舒张末压值(LVEDP),表明藜麦蛋白可以有效改善大鼠力竭运动的血流动力学变化。藜麦蛋白由于具有较强的抗氧化能力,可以起到降低丙二醛(MDA)水平、提高超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)等指标的作用,表明藜麦蛋白可以改善心脏和机体的氧化应激系统。

Response surface methodology was used to optimize the enzymatic extraction of quinoa protein,and its effects on hemodynamics and oxidative stress in exhaustive exercise rats were studied. The optimum extraction process of quinoa protein was as follows: liquid material ratio 21 ∶ 1( m L/g),extraction temperature 52 ℃,enzyme addition 4. 2 %( based on the quality of quinoa powder). Under these conditions,the average extraction rate of quinoa protein was 71. 26 %,which was little different from the theoretical prediction. Rat exhaustive exercise test showed that quinoa protein could increase left ventricular systolic pressure( LVSP) and reduce left ventricular end diastolic pressure( LVEDP),indicating that quinoa protein could effectively improve the hemodynamic changes of rats after exhaustive exercise.Due to its strong antioxidant capacity,quinoa protein can reduce the level of malondialdehyde( MDA)and improve the indexes of superoxide dismutase( SOD) and glutathione peroxidase( GSH-Px),indicating that quinoa protein can improve the oxidative stress system of heart and body.