月季优质品系的高效繁殖体系研究

Study on high efficient propagation system of Rosa chinensis‘Red and Pink’

以古老月季优质品系的小叶及复叶柄为外植体,探讨了月月粉(Rosa chinensis‘Pink China’)自交后代一优良品系月月红粉(Rosa chinensis‘Red and Pink’)的愈伤组织诱导、体细胞胚胎发生与增殖及植株高效再生的方法。结果表明,2,4-D可能是月月红粉最适宜愈伤诱导的生长激素;最佳外植体为复叶柄;同时添加L-p和AgNO_(3)对体细胞胚发生有促进作用;在MS+(3.0,4.0,5.0,6.0 mg/L)2,4-D+400 mg/L L-p+10 mg/L AgNO_(3)+3%Glucose+3 g/L GEL培养基上,外植体最初形成愈伤组织,经过16周培养后均不同程度发生体细胞胚;适合月月红粉体细胞胚增殖的培养基为:MS+1.0 mg/L 2,4-D+0.05 mg/L BA+3%Glucose+0.3%GEL。体细胞胚在萌发培养基1/2 MS+3%Glucose+0.3%GEL+1.0 mg/L BA+0.01 mg/L NAA上萌发培养,最终建立了以月月红粉体细胞胚发生途径为基础的高效再生体系,为加快蔷薇属植物的功能基因验证及相应月季分子育种进度奠定了基础。

This study describes an effective regeneration system via calli induction,somatic embryogenesis and embryos germination using leaflets and compound leaf as explants in Rosa chinensis‘Red and Pink’.The results showed that,high-frequency primary so⁃matic embryos inductions were obtained from petioles of compound leaf combining 2,4-D,that L-p and as AgNO_(3) promoted somatic embryogenesis.When explants were incubated on MS medium supplemented with various concentration of 2,4-D(3.0,4.0,5.0,6.0 mg/L)respectively,added 400 mg/L L-p and 10 mg/L AgNO_(3) and solidified using 0.3%Gel,after 16 weeks culture,they firstly formed embryogenic calli and finally somatic embryos.MS medium containing 1.0 mg/L 2,4-D,0.05 mg/L 6-BA and 3%glucose was found to be the most effective in the proliferation of somatic embryos.After germination culture on medium(1/2 MS+3%Glucose+0.3%GEL+1.0 mg/L BA+0.01 mg/L NAA),a somatic embryogenesis based regeneration protocol were developed and it will be signifi⁃cant to accelerate the propagation and extensive utilization of Rosa genus species.