血管紧张素Ⅱ皮下埋泵建立ApoE^(-/-)小鼠主动脉夹层模型

Establishment of Aortic Dissection Model in ApoE^(-/-)Mice by Subcutaneous Embedding of Angiotensin Ⅱ Pump

目的应用血管紧张素Ⅱ(angiotensin,AngⅡ)皮下埋泵建立ApoE^(-/-)小鼠主动脉夹层模型。方法选取30只25~28周龄雄性ApoE^(-/-)小鼠随机分为对照组和实验组各15只,分别通过皮下埋藏植入式微量渗透缓释泵皮下输入AngⅡ[2500ng/(kg·min)]和渗透量为0.5μl/h的0.9%氯化钠注射液,持续泵入7天。采用无创血压计检测小鼠血压和心率并且记录小鼠每天的饮食饮水量。处死小鼠前,使用小动物超声影像系统观察小鼠有无动脉夹层形成,随后安乐死处死小鼠取材,固定包埋组织做石蜡切片并且进行HE染色及EVG染色,显微镜下观察主动脉弹性纤维撕裂和假腔形成情况。结果实验组有2只小鼠因主动脉破裂出血死亡,其余小鼠主动脉夹层发生率为62%(8/13),显微镜下观察可见动脉血管壁增厚弹性纤维紊乱,有假腔形成;而对照组小鼠实验过程中未出现死亡且未观察到主动脉夹层形成(0/15)。结论使用本方法能够成功,有效地建立ApoE^(-/-)小鼠主动脉夹层模型。

Objective To establish a mouse model of aorta dissection(AD)by subcutaneously AngⅡinfusion into Apo E-/-mouse.Methods Five month-old male Apo E-/-mice subcutaneous infused with angiotensinⅡthrough an implantable osmotic pump for 7 days at a rate of 2500ng/(kg·min).The control group was infused with 0.9%saline at a rate of 0.5μl/h.The blood pressure and heart rate of the mice were measured by non-invasive sphygmanometer and the dietary water intake of mice was recorded every day.Before the mice were killed,the formation of arterial dissection was observed by small animal ultrasound imaging system.Then,the mice were euthanized and sacrificed.The embedded tissue was fixed for paraffin section and HE staining and EVG staining were performed.Results In the experimental group,2 mice died due to aortic rupture,and the incidence of aortic dissection in the remaining mice was 62%(8/13).Microscope observation showed that arterial wall was thickened and elastic fiber disorder and false lumen was formed.In contrast,no death or aortic dissection was observed in control mice(0/15).Conclusion This method can successfully,efficiently establish ApoE^(-/-)mice aortic dissection model.