湿寒应激对绝经大鼠骨生物力学及尿骨代谢指标的影响

Effects of Dampness-cold Stress on Bone Biomechanics and Urinary Indexes in Menopausal Rats

目的通过检测湿寒应激条件下绝经大鼠骨密度、骨生物力学及尿生化指标,探讨其对骨质疏松的影响及意义。方法选用SD雌性大鼠48只,随机分为正常对照组、假手术组、OVX模型组和OVX+湿寒组,每组12只,OVX+湿寒组给予应激条件干预9周,于术后7周、9周测量大鼠骨密度、骨生物力学及尿生化指标。结果(1)骨密度(BMD):第7周,与OVX模型组比较,OVX+湿寒组降低。(2)骨生物力学:与正常对照组及假手术组比较,OVX+湿寒组骨折应力、杨氏模量明显降低。(3)尿生化指标:第7周,与正常对照组及假手术组比较,OVX模型组及OVX+湿寒组尿钙降低、尿磷升高(P<0.05);与OVX模型组比较,OVX湿寒组尿钙、尿磷、尿NTX、尿CTX升高(P>0.05)。结论湿寒应激可使绝经大鼠骨密度降低,骨折应力及杨氏模量下降,可能与改变骨骼钙磷代谢及加速骨吸收有关。

Objective To investigate the effect and significance of bone mineral density,bone biomechanics and urinary biochemical indexes of menopausal rats under dampness-cold stress.Methods Forty-eight female SD rats were randomly divided into normal control group,sham operation group,OVX model group and OVX+dampness-cold group,with 12 rats in each group.The OVX+dampness-cold group treated with stress condition intervention for 9 weeks.The bone mineral density,bone biomechanics and urinary biochemical indexes of the rats were measured at 7 and 9 weeks after surgery.Results(1)Bone mineral density(BMD):at week 7,compared with OVX model group,OVX+dampness-cold stress group decreased.(2)Bone biomechanics:Compared with normal control group and sham operation group,fracture stress and Young′s modulus of OVX+dampness-cold group were significantly decreased.(3)Urinary biochemical indicators:at week 7,compared with normal control group and sham operation group,urinary calcium in OVX model group and OVX+dampness-cold group decreased and urinary phosphorus increased(P<0.05);Compared with OVX model group,urine calcium,urine phosphorus,urine NTX and urine CTX were increased in OVX dampness-cold group(P>0.05).Conclusion Dampness-cold stress can decrease bone density,fracture stress and Young′s modulus in menopausal-induced osteoporosis rats,which may be related to the change of bone calcium and phosphorus metabolism and the acceleration of bone resorption.