长链非编码RNA ZEB2-AS1在非小细胞肺癌中的表达及临床病理特征与预后的关系

Expression of LncRNA ZEB2-AS1 in Non-small Cell Lung Cancer and its Relationships with Clinicopathological Features and Prognosis

目的探讨长链非编码核糖核酸(lncRNA)锌指E盒结合同源盒蛋白2-AS1(lncRNA ZEB2-AS1)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达水平,并分析其与患者临床病理特征及预后的关系。方法选取笔者医院2013年1月~2018年3月行手术治疗的NSCLC患者129例,实时荧光定量聚合酶链反应(qRT-PCR)检测肺癌组织和癌旁正常肺组织中lncRNA ZEB2-AS1表达,并收集患者临床病理资料,分析其与患者临床病理特征关系。对肺癌患者预后进行随访,采用Kaplan-Meier法分析lncRNA ZEB2-AS1表达与患者预后关系。采用COX比例风险模型分析影响肺癌患者预后的相关因素。结果与癌旁正常肺组织比较,NSCLC组织中lncRNA ZEB2-AS1的表达水平显著升高(P<0.001)。lncRNA ZEB2-AS1的表达水平与NSCLC患者是否发生淋巴结转移、肿瘤分化程度、TNM分期显著相关(P<0.001)。lncRNA ZEB2-AS1高表达组总生存期显著低于低表达组(34.5%vs 70.2%,P=0.013)。COX多因素分析显示分化程度、淋巴转移、TNM分期、lncRNA ZEB2-AS1表达均是影响NSCLC患者预后的独立危险因素(P<0.05)。结论lncRNA ZEB2-AS1在NSCLC组织中呈高表达,与淋巴结转移、肿瘤分化程度、TNM分期显著相关,是影响患者预后的独立危险因素,可将其作为NSCLC患者早期诊断的标志物及治疗靶点。

Objective To investigate the expression of long non-coding ribonucleic acid(lncRNA)zinc finger E-box binding homeobox protein 2-AS1(lncRNA ZEB2-AS1)in non-small cell lung cancer,and to analyze its relationships with clinicopathological characteristics and prognosis.Methods A total of 129 patients with NSCLC who underwent surgical treatment in our hospital from January 2013 to March 2018 were selected as the research objects.The NSCLC tissues and adjacent normal lung tissues were detected for the expression levels of lncRNA ZEB2-AS1 by the real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).The clinicopathological data of the patients were collected to analyze the relationship between the clinicopathological characteristics of the patients and lncRNA ZEB2-AS1.Prognosis of patients with NSCLC were followed-up.The relationship between the expression of lncRNA ZEB2-AS1 with the prognosis of patients was analyzed by Kaplan-Meier method.COX proportional hazard model was used to analyze the prognostic factors of NSCLC patients.Results The expression level of lncRNA ZEB2-AS1 was significantly up-regulated in NSCLC tissues compared with matched adjacent lung tissues(P<0.001).The expression level of lncRNA ZEB2-AS1 was significantly correlated with lymph node metastasis,tumor differentiation and TNM stage in patients with NSCLC cancer(P<0.05).The overall survival of the patients in the high expression group of lncRNA ZEB2-AS1 was significantly lower than that in the low expression group(34.5%vs 70.2%,P=0.013).COX multivariate analysis showed that lymph node metastasis,tumor differentiation,TNM stage and lncRNA ZEB2-AS1expression were independent risk factors affecting the prognosis of NSCLC patients(P<0.05).Conclusion LncRNA ZEB2-AS1 is significantly increased in NSCLC tissues,and is correlated with lymph node metastasis,tumor differentiation and TNM stage,and is an independent risk factor affecting the prognosis of patients.It may serve as a marker for early diagnosis and therapeutic target of NSCLC.