鸡传染性喉气管炎实验室诊断与序列分析

Laboratory Diagnosis and Sequence Analysis of Infectious Laryngotracheitis Virus

从山东省东营市某养鸡企业采集具有呼吸道症状及产蛋障碍的蛋鸡组织共8份,经病史与免疫程序调查、病鸡的临床症状与组织病理变化,初步怀疑是传染性喉气管炎、传染性支气管炎或低致病性禽流感病毒感染。通过病毒血凝试验排除了低致病性禽流感病毒;通过PCR方法确定为传染性喉气管炎病毒感染;最终对扩增的病毒gD基因与TK基因进行测序并与BLAST比对分析,发现该病毒与疫苗毒株同源性高达99%。以上结果表明,传染性支气管炎疾病的免疫接种仍然重要。

A total of 8 samples of layers with respiratory symptoms and egg production disorders were collected from a chicken enterprise in Dongying city, Shandong Province.We suspected that the chickens were infected with infectious laryngotracheitis virus,infectious bronchitis virus or low pathogenic avian influenza virus via the clinical symptoms and histopathological changes. Low pathogenic avian influenza virus was excluded by virus hemagglutination test. It was identified as infectious bronchitis virus infection by PCR test. Sequencing and BLAST analysis showed that gD and TK genes had 99% homology with the vaccine strain. These results indicate that immunization against infectious bronchitis is still important.