消退素D1对大鼠肝缺血再灌注损伤的保护作用

The protective role of RvD1 in a rat model of liver ischemia-reperfusion injury

目的探讨消退素D1(resolvin D1,RvD1)对大鼠肝缺血再灌注损伤(ischemia reperfusion injury,IRI)的作用。方法实验动物随机分为假手术组(Sham组)、IR组、RvD1组和ZnPP组。自动生化仪检测氨基转移酶水平;苏木精-伊红染色观察肝组织学变化;髓过氧化物酶(myeloperoxidase,MPO)试剂盒测定MPO水平;实时荧光定量聚合酶链反应(quantitative real-time PCR,qRT-PCR)检测细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)水平。结果与IR组相比,RvD1组大鼠ALT(P<0.05)、AST(P<0.01)明显降低,肝组织IRI减轻,肝内MPO活性降低(P<0.05),ICAM-1 mRNA表达下降(P<0.05)。而ZnPP组则与RvD1组表现相反。结论RvD1通过降低MPO活性及ICAM-1 mRNA表达发挥对大鼠肝IRI的保护作用,且可以被ZnPP所逆转。

Objective To investigative the effect of resolvin D1(RvD1)in the rat's liver IRI and the possible mechanism.Methods SD rats were divided into 4 groups randomly:Sham group,IR group,RvD1 group(IR+RvD1)and ZnPP group(IR+RvD1+ZnPP).The levels of ALT and AST in the serum were detected with biochemical analyzer.MPO in the rat's liver were detected with MPO kit.The changes of liver cell were observed with H-E staining.The mRNA level of ICAM-1 in rat's liver were tested with quantitative real-time PCR(qRT-PCR).Results In RvD1 group,the levels of ALT(P<0.05)and AST(P<0.01)were decreased significantly.The swellings and necrosis in the rat liver cells in RvD1 group were observed to alleviate through H-E staining.Compared with IR group,the level of MPO in RvD1 group was reduced by MPO kit.And the mRNA level of ICAM-1 in RvD1 group was measured to decrease by qRT-PCR.Conclusion RvD1 attenuates the IRI in rat's liver by reducing the level of MPO and ICAM-1 and the protective role is reversed by the inhibitor of HO-1,ZnPP.