基于氮掺杂荧光碳量子点探针的制备及其应用

Preparation and application of nitrogen-doped fluorescent carbon quantum dot probe

以柠檬酸为碳源,乙二胺为氮源,采用水热法一步成功合成氮掺杂荧光碳量子点(N-CQDs)探针。系统性的研究掺杂比例、反应温度以及反应时间对N-CQDs光学性能的影响,并利用透射电子显微镜、傅里叶红外光谱法、紫外吸收光谱法和荧光光谱法等技术对N-CQDs结构进行表征。作为新型荧光探针,发现二价汞离子(Hg^(2+))对该碳量子点具有很好的选择性猝灭作用,由此构建高灵敏检测痕量Hg^(2+)的荧光猝灭新方法,可用于实际环境水样中Hg^(2+)的检测分析。

Nitrogen-doped fluorescent carbon quantum dots(N-CQDs)probe was successfully synthesized by hydrothermal method using citric acid as carbon source and ethylenediamine as nitrogen source.The effects of doping ratio,reaction temperature and reaction time on the optical properties of N-CQDs were systematically studied,and the structure of N-CQDs was characterized by transmission electron microscopy,Fourier infrared spectroscopy,ultraviolet absorption spectroscopy and fluorescence spectroscopy.As a new fluorescent probe,divalent mercury ion(Hg^(2+))was found to have a good selective quenching effect on the carbon quantum dots.Therefore,a new fluorescence quenching method with high sensitivity for the detection of trace Hg^(2+)was established,which can be used for the detection and analysis of Hg^(2+)in actual environmental water samples.