牡丹TCP家族基因的鉴定与生物信息学分析

Identification and Bioinformatics Analysis of TCP Family Genes in Tree Peony

TCP(Teosinte branched1/Cincinnata/proliferating cell factor)家族基因是植物特有的一类转录因子,在植物生长发育的过程中具有重要的调控作用。本研究从牡丹转录组数据中鉴定出18个TCP基因,分子量在18978.32~51789.56 Da之间,等电点在5.81~9.45之间,TCP家族成员亚细胞定位预测均在细胞核中。牡丹TCP结构域中保守氨基酸的分类与拟南芥相似,系统发育分析结果表明,牡丹TCP蛋白分为两大类,ClassⅠ包含10个TCP成员,ClassⅡ包含8个TCP成员。MEME分析结果显示,在牡丹TCP蛋白中发现了15个保守结构域,其中motif 1为TCP结构域,在所有TCP成员中都存在。同时,对牡丹TCP基因在芽、叶、花瓣、花斑、雄蕊、雌蕊和种子7个组织中的转录水平进行了分析。本研究对牡丹TCP家族基因进行了系统的分析,为进一步开展牡丹功能基因研究及分子育种提供了科学依据。

The TCP(Teosinte branched1/Cincinnata/proliferating cell factor)family gene is a kind of plant specific transcription factor,which plays a significant role in regulating plant growth and development.In this study,18 TCP genes were identified from the tree peony transcriptome data,the molecular weight was between 18978.32 and51789.56 Da,the isoelectric points were between 5.81 and 9.45,and the subcellular localization prediction of TCP family members was in the nucleus.The classification of conserved amino acids in the tree peony TCP domain was similar to that of Arabidopsis thaliana.Phylogenetic analysis showed that the TCP proteins of tree peony were divided into two categories,ClassⅠcontained 10 TCP members,and ClassⅡcontained 8 TCP members.MEME analysis results showed that 15 conserved domains were found in the tree peony TCP protein,motif 1 was the TCP domain,and was found in all TCP members.Meanwhile,the transcription level of the tree peony TCP genes in bud,leaf,petal,piebaldness,stamen,pistil and seed were analyzed.This study systematically analyzed the tree peony TCP family genes,which provided a scientific basis for further research on functional genes and molecular breeding in tree peony.