香蕉TCP家族的全基因组鉴定及对低氮胁迫的响应

Genome-wide Identification of Musa acuminata TCP Family and Its Response to Low Nitrogen Stress

TCP(Teeosinte branchedⅠ,Cycloidea,Proliferating cell fatcors 1和2)是含有一个高度保守非典型的bHLH(basic-Helix-Loop-Helix)保守结构域的植物特有转录因子基因家族之一,参与调控植物生长发育和非生物胁迫响应等生理过程。为了探究香蕉(Musa acuminata)TCP基因家族对低氮胁迫的响应,本研究从全基因组的分布、蛋白质理化性质、保守结构域等生物信息学方面阐述了香蕉MaTCPs基因家族成员的基本特征,并且对香蕉叶片和根在低氮胁迫下的MaTCPs基因家族成员的表达模式进行了分析。结果表明,香蕉TCP转录因子家族共包含48个成员,可分为ClassⅠ(PCF)和ClassⅡ(CYC/TB1和CIN)两个亚家族,所有成员均定位于除Chr.09和chrUn_random以外的染色体上,且存在多个串联重复基因对。在低氮胁迫下,香蕉MaTCPs基因家族大部分基因表达量上调,且在不同的组织中表达模式不同。同一亚家族基因在根和叶中表现出一致的表达模式。这些结果表明Ma TCPs家族成员可能参与了香蕉对于低氮胁迫的响应。本研究将为香蕉低氮胁迫的机制研究和香蕉TCP转录因子家族的功能分析提供理论基础,为香蕉抗逆育种提供参考。

TCP(Teeosinte branchedⅠ,Cyclidea,Proliferating cell fatcors 1 and 2)is one of the plant-specific transcription factor gene families containing a highly conserved atypical bHLH(basic-Helix-Loop-Helix)conserved domain,which is involved in regulating physiological processes such as plant growth and development and abiotic stress response.In order to explore the response of the banana(Musa acuminata)TCP gene family to low nitrogen stress,this study explained the basic characteristics of the members of the banana MaTCPs gene family from the bioinformatics aspects of the whole genome distribution,physicochemical properties of protein and conserved domains.In addition,the expression patterns of members of the Ma TCPs gene family in banana leaves and roots under low nitrogen stress were analyzed.The results showed that the banana TCP transcription factor family contained 48 members,which were be divided into two sub-families,ClassⅠ(PCF)and ClassⅡ(CYC/TB1 and CIN).All members were located on chromosomes other than Chr.09 and chrUn_random,and there were multiple tandem repeat gene pairs.Under low nitrogen stress,the expression of most genes in the banana MaTCPs gene family were up-regulated,and the expression patterns were different in different tissues.Genes of the same subfamily showed consistent expression patterns in roots and leaves.These results indicated that members of the MaTCPs family may be involved in the response of bananas to low nitrogen stress.This study will provide a theoretical basis for the study of the mechanism of banana low nitrogen stress and the functional analysis of banana TCP transcription factor family.It will provide a reference for banana resistance to reverse breeding.