彰武松离体培养初步分析

Preliminary Study on In Vitro Culture of Pinus densiflora var.zhangwuensis

以彰武松休眠芽和次年新梢为外植体,初步建立彰武松离体培养研究体系。结果表明:以当年发的新梢作为外植体,经1.5%H_(2)O_(2)预处理消毒10 min,75%乙醇30 s,0.1%Hg Cl_(2)消毒6 min消毒效果最好;接种在DCR+6-BA 2 mg/L+NAA 0.2 mg/L培养基中,能够诱导外植体顶部长出幼嫩的2针1束针叶,且生长旺盛,颜色鲜绿,活力强,诱导率达48.7%。以彰武松针叶为外植体进行体胚诱导,将消毒后的针叶接种在DCR+2,4-D 5 mg/L+NAA 5 mg/L的培养基中培养,能够产生愈伤组织,愈伤组织诱导率为100%,获得的愈伤组织为非胚性愈伤组织。

A preliminary study system of tissue culture of Pinus densiflora var.zhangwuensis was established by using dormant buds and shoots of the next year as explants.The results showed that the new shoots from the same year as explants had the best disinfection effect after pretreatment with 1.5%H_(2)O_(2)for 10 min,75%ethanol for 30 s,and 0.1%Hg Cl_(2)for 6 min.Inoculated in DCR+6-BA 2 mg/L+NAA 0.2 mg/L medium,the explants could be induced to sprout two young needles and a bunch of needles at the top of the explants with vigorous growth,bright green color and strong vitality,and the induction rate reached 48.7%.Somatic embryo induction was conducted on the pine needles as explants,and the sterilized needles were cultured in the medium of DCR+2,4-D 5 mg/L+NAA5 mg/L,which could produce callus.The callus induction rate was 100%,and the obtained callus were non-embryonic callus.