外周血中肺腺癌转移相关转录本1对口腔鳞状细胞癌的临床诊断价值

Clinical diagnostic value of metastasis-associated lung adenocarcinoma transcript 1 in peripheral blood of patients with oral squamous cell carcinoma

目的:探讨外周血中肺腺癌转移相关转录本1(metastasis-associated lung adenocarcinoma transcript 1,MALAT1)对口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)的临床诊断价值。方法:采用荧光定量RT-qPCR方法检测OSCC患者术前外周血样本及配对术后癌组织中MALAT1的表达。结果:MALAT1在OSCC患者外周血中的平均表达水平明显高于健康志愿者(1.845±0.142 vs. 0.642±0.090,P<0.001);MALAT1在外周血中的表达水平与配对癌组织成正相关,相关系数为0.783(P<0.001);MALAT1在外周血中的表达水平与肿瘤TNM分期(P=0.001)及细胞分化程度相关(P=0.010);MALAT1对OSCC诊断的ROC曲线下面积为0.892,当截断值为0.788时,其诊断灵敏度为85%,特异度为81%。结论:MALAT1对OSCC具有较好的临床诊断价值,有望成为OSCC疾病诊断、预后判断的血清分子标志物。

Objective:To investigate the clinical diagnostic value of metastasis-associated lung adenocarcinoma transcript 1(MALAT1) in the peripheral blood of patients with oral squamous cell carcinoma(OSCC). Methods:Fluoresence quantitative RT-qPCR was used to detect the expression of MALAT1 in the preoperative peripheral blood samples and paired postoperative tumor tissues of patients with OSCC. Results:The mean expression level of MALAT1 in peripheral blood of patients with OSCC was significantly higher than that of healthy volunteers(1.845±0.142 vs. 0.642 ±0.090,P<0.001). The expression level of MALAT1 in peripheral blood was positively correlated with the paired tumor tissues with a correlation coefficient of 0.783(P<0.001). The expression level of MALAT1 in peripheral blood was correlated with tumor TNM stage(P =0.001) and cell differentiation(P =0.010). The area under the ROC curve of MALAT1 for the diagnosis of OSCC was 0.892. When the cutoff value was 0.788,the diagnostic sensitivity and specificity of MALAT1 were 85% and 81% respectively. Conclusion:MALAT1 has potential clinical value of diagnosing OSCC,and is expected to be a serum molecular marker for the diagnosis and prognosis of OSCC.