两种国产荧光定量PCR试剂检测HBV DNA载量的对比分析

HBV DNA load detected by two domestic fluorescent quantitative PCR reagents:a comparative analysis

目的比较2种国产实时荧光定量PCR试剂在检测乙型肝炎病毒HBV DNA的定量检测结果。方法抽取306份乙型肝炎患者的血清标本,采用国产试剂A与B进行高敏HBV DNA定量平行检查,以对2种HBV DNA定量试剂进行结果差异和一致性分析。结果1)试剂A检出率为86.92%,试剂B检出率为84.64%,回归直线方程Y=0.9849x+0.1549,R2=0.9457,相关系数r=0.9725,提示2种荧光定量PCR试剂检测HBV DNA结果相关性良好。2)浓度在(20~1000)IU/mL范围内,试剂A和试剂B的检出率分别为39.9%和37.6%。结论试剂A更适用OBI和HBeAg(-)患者的治疗后期病情监测,亦可在开展其他手术前/输血前开展对患者HBV病原体检测。

Objective To compare the quantitative detection results of two domestic quantitative real-time PCR reagents in HBV-DNA detection.Methods A total of 306 serum samples form hepatitis B patients were selected for quantitative parallel detection of high-sensitiveity HBV-DNA using domestic reagent A and B,and the difference and consistency of the results were analyzed.Results 1)The yielding rate of reagent A and B was 86.92%and 84.64%,respectively.The regression linear equation was Y=0.9849 x+0.1549,R2=0.9457,the correlation coefficient r=0.9725,indicating the results by the two reagents had good correlation.2)When the concentration was in the range of(20~1000)IU/mL,the yielding rates of reagent A and B were 39.9%and 37.6%,respectively.Conclusion Reagent A is more suitable for post-treatment monitoring in patients with OBI and HBeAg(-),but also can be used to detect HBV pathogens in patients before operations or blood transfusions.