两种容量冷沉淀的质量对比分析

Quality comparison of cold cryoprecipitates in two different volumes

目的研究25 mL和45 mL冷沉淀中凝血因子Ⅷ(FⅧ)和纤维蛋白原(Fg)浓度、含量及得率的对比分析。方法选取40份200 mL新鲜全血样本分为A组和B组,每组各20份样本,在6 h(抗凝剂ACD)内按照成分制备标准操作规程制备出新鲜冰冻血浆(FFP),1周后将制备的FFP用虹吸法原理制备成25±5 mL和45±5 mL冷沉淀,冰冻1周后在37℃水浴箱融化后检测FⅧ和Fg浓度并计算含量和得率。结果 A组和B组的FVIII浓度分别为(2.990±0.988)和(2.744±0.940)IU/mL,含量分别为(74.75±24.71)和(113.75±30.06)IU,得率分别为(70.1±16.6)和(85.0±7.6)%;Fg的浓度分别为(6.013±1.679)和(5.844±0.683)g/L,含量分别为(150.33±41.99)和(252.23±26.90)mg,得率分别为(41.7±8.6)和(49.1±9.6)%,经统计学分析,两组的FⅧ含量和得率有统计学差异(P<0.05),Fg的含量和得率有统计学差异(P<0.05),FⅧ和Fg的浓度无统计学差异(P>0.05)。结论低容量冷沉淀FⅧ和Fg的含量和得率较低,浓度略高,两种容量产品都能达到全血及成分血质量要求,从临床病人液体输注量方面考虑,建议200 mL全血制备冷沉淀时,适当降低产品容量。

Objective To study the concentration, content and yield of coagulation factor Ⅷ(FⅧ) and fibrinogen(Fg) in 25 mL and 45 mL cryoprecipitate.Methods Forty aliquots(200 mL fresh whole blood) were divided into group A and group B, with 20 samples in each group. Fresh frozen plasma(FFP) was prepared within 6 hours(anticoagulant ACD) according to the standard operating procedure for component preparation. After one week, the prepared FFP was prepared into(25±5) mL and(45±5) mL cold precipitates by siphon method. After freezing for one week, the concentrations of FⅧ and Fg were detected after meltingin by water bath at 37℃, and the content and yield were calculated. Results The FⅧ concentration, content and yield in group A and group B were(2.990±0.988) vs(2.744±0.940) IU/mL,(74.75±24.71) vs(113.75±30.06)IU, and(70.1±16.6) vs(85.0±7.6)%, respectively.The Fg concentration, content and yield was(6.013±1.679) vs(5.844±0.683) g/L,(150.33±41.99) vs(252.23±26.90)mg, and(41.7±8.6) vs(49.1±9.6)%, respectively. Statistical analysis suggested that the content and yield of FⅧ and Fg were statistically different between the two groups(P<0.05), but the concentration of FⅧ and Fg was not statistically different(P>0.05). Conclusion FⅧ and Fg of low-volume cryoprecipitate presented lower content and yield, but slightly higher concentration. Both products can meet the quality requirements of whole blood and blood component. Therefore, reducing product capacity appropriately is suggested when preparing cryoprecipitate with 200 mL whole blood.