2021年福建省龙岩市病死鸡群中4种病毒感染情况调查

Investigation on the Prevalence Status of Four Kinds of Viruses in Dead Chickens in Longyan City of Fujian Province in 2021

为探明2021年龙岩市规模化鸡场鸡传染性贫血病病毒(CIAV)、圆圈病毒3型(GyV3)、鸡细小病毒(ChPV)、J亚群禽白血病病毒(ALV-J)等4种病毒的感染情况,对龙岩市32个规模化鸡场采集的297份病死鸡组织样品进行PCR或RT-PCR检测,并对检测结果进行统计分析。结果显示:CIAV、GyV3、ChPV和ALV-J均有检出,阳性检出率分别为34.34%、28.62%、18.86%、13.13%;从病原分布看,CIAV分布最广,除漳平市外其余6个区县均有检出,各区县检出率介于12.00%~98.00%;其他3种病原呈局限性分布,均只在4个区县有检出。共检出单一感染阳性样品79份,总单一感染阳性检出率为26.60%(79/297),单一感染阳性检出率从高到低依次为Ch PV(14.81%)、CIAV(5.05%)、ALV-J(4.71%)、Gy V3(2.02%)。CIAV+Ch PV双重感染阳性检出率为2.69%(8/297),CIAV+GyV3为18.18%(54/297);CIAV+ALV-J+GyV3三重感染阳性检出率为7.07%(21/297),CIAV+ChPV+ALV-J+GyV3四重感染为1.34%(4/297)。结果表明:龙岩市鸡群中存在CIAV、ALV-J、ChPV和GyV34种病原的感染,且部分地区感染较为严重,混合感染现象普遍,提示龙岩市应积极开展疫病监测,加强鸡场的生物安全综合防控。

In order to investigate the prevalence status of four kinds of viruses including chicken infectious anemia virus(CIAV),gyrovirus 3(GyV3),chicken parvovirus(Ch PV)and avian leukaemia virus subgroup J(ALV-J)in intensive farms in Longyan City in 2021,297 tissue samples collected from dead chickens in 32 intensive farms in the city were tested,from which the results were summarized and analyzed. The results showed that all the above viruses were detected out,and the positive rates of CIAV,GyV3,ChPVl,ALV-J were 34.34%,28.62%,18.86% and 13.13% respectively;for the distribution of pathogens,CIAV was the most widespread,and detected from six districts/counties except Zhangping City,and the detection rate of each region ranged within 12.00% to 98.00%;the other three pathogens were localized and detected in four districts/counties. A total of 79 positive samples with single infection were detected,the detection rate was ChPV(14.81%),CIAV(5.05%),ALV-J(4.71%)and GyV3(2.02%)from high to low,and the total rate was 26.60%(79/297). The detection rates of double infection were 2.69%(8/297)for CIAV+ChPV,and 18.18%(54/297)for CIAV+GyV3;the detection rate of triple infection was 7.07%(21/297)for CIAV+ALV-J+GyV3,and the detection rate of quadruple infection was 1.34%(4/297). In conclusion,CIAV,ALV-J,Ch PV and GyV3 were prevalent in the chickens in Longyan City,and particularly more serious in some regions where mixed infection was usually detected,against which,monitoring on epidemic diseases should be actively carried out,meanwhile comprehensive prevention and control bio-safety measures should be strengthened in the farms.