香烟烟雾暴露对小鼠糖代谢的影响及穿心莲内酯的干预效果

Influences of cigarette smoke exposure on glucose metabolism in mice and intervention effect of andrographolide

目的探讨香烟烟雾暴露对小鼠糖代谢的影响及穿心莲内酯的干预效果,为糖尿病预防及烟草控制提供依据。方法选择SPF级C57BL/6J品系雄性小鼠60只,按照小鼠体重随机分为正常对照组(A组)、烟雾暴露组(B组)和烟雾暴露+穿心莲内酯干预组(C组),每组20只。B组和C组小鼠每天暴露于20支香烟的烟雾60 min,持续3个月;C组小鼠每周腹腔内注射穿心莲内酯(10 mg/kg),A组和B组注射同等体积生理盐水。香烟烟雾暴露结束后施行腹腔内注射葡萄糖耐量实验(IPGTT)和胰岛素耐量实验(IPITT),比较各组小鼠各时点血糖变化及IPGTT和IPITT血糖水平的曲线下面积。结果0 min时,B组的血糖最高,C组次之。IPGTT示,腹腔注射葡萄糖后各组小鼠的血糖水平迅速增加,注射后15 min最高,随后逐渐下降,且B组在15 min和30 min时的血糖水平均显著高于A组(P<0.01);B组和C组血糖水平的曲线下面积均显著大于A组(P<0.05),B组显著大于C组(P<0.05)。IPITT示,腹腔注射胰岛素后,15 min时B组血糖水平显著高于A组(P<0.05),30 min和60 min时各组小鼠的血糖水平及血糖曲线下面积差异无统计学意义(P>0.05)。结论香烟烟雾可能主要通过影响胰岛素分泌功能,引起小鼠糖代谢的异常,而穿心莲内酯可以拮抗这种损伤。但未发现香烟烟雾对小鼠的胰岛素敏感性有明显影响。

Objective To investigate the impacts of cigarette smoke(CS)exposure on glucose metabolism in mice and the intervention effect of andrographolide to provide evidence for diabetes prevention and tobacco control.Methods Sixty SPF male mice of C57BL/6J strain were selected and randomly divided into normal control group(group A),CS exposure group(group B)and CS exposure plus andrographolide intervention group(group C)according to their body weight(n=20,each).The mice in group B and C were exposed to 20 cigarettes smoke for 60 minutes every day for 3 months.Mice in group C were injected with andrographolide(10 mg/kg)intraperitoneally every week,and mice in group A and group B were injected with the same volume of normal saline.After CS exposure,intraperitoneal glucose tolerance tests(IPGTT)and intraperitoneal insulin tolerance tests(IPITT)were performed to compare the changes in blood glucose and the area under the curve(AUC)of blood glucose levels of IPGTT and IPITT among three groups at each time point.Results The blood glucose level was the highest in group B,followed by group C at 0 min.After intraperitoneal injection of glucose,the blood glucose levels in three groups increased rapidly and were the highest at 15 minutes after injection,and then they gradually decreased.The blood glucose levels in group B were significantly higher than those in group A at 15 min and 30 min(P<0.01).The AUC of blood glucose levels in group B and group C was significantly larger than that in group A(P<0.05),and the AUC in group B was significantly larger than that in group C(P<0.05).After intraperitoneal injection of insulin,the blood glucose level in group B was significantly higher than that in group A at 15 min,and there were no significant differences in the blood glucose levels at 30 min and 60 min among three groups.Also,there was no significant difference in AUC of IPITT among three groups(P>0.05).Conclusion Cigarette smoke may cause abnormal glucose metabolism in mice by affecting insulin secretion,and andrographolide can antagonize this damage,but it is not found that cigarette smoke exposure has a significant effect on insulin sensitivity in mice.