高效液相色谱法测定防老剂TMQ有效成分含量的不确定度评定

Evaluation of Uncertainty in Determination of Active Component Content of Antioxidant TMQ by HPLC

对高效液相色谱法测定防老剂TMQ的主要有效成分2,2,4-三甲基-1,2-二氢化喹啉的二、三、四聚体(简称二、三、四聚体)总含量的不确定度进行评定。建立测定防老剂TMQ的二、三、四聚体总含量的数学模型,分析测量过程中各不确定度来源,包括称取样品质量、容量瓶定容、测量重复性和仪器测量引入的不确定度,并对各个不确定度分量进行量化,得到测量结果的合成标准不确定度与扩展不确定度。

The uncertainty of total content of dimer,trimer and tetramer of 2,2,4-trimethyl-1,2-dihydroquinoline(referred to as dimer,trimer and tetramer),the main active component of antioxidant TMQ,was evaluated by high-performance liquid chromatography(HPLC).A mathematical model for determining the total content of dimer,trimer and tetramer of antioxidant TMQ was established.The sources of uncertainty in the test process,including uncertainties introduced by weight of the sample,the constant volume of the volumetric flask,the measurement repeatability and the instrument were analyzed.Each uncertainty component was quantified,and the combined standard uncertainty and extended uncertainty of the measurement results were obtained.