两种混合STR分型分析软件的比较与应用

Comparison and Application of Two Pieces of Software for Interpretation of Mixed STR Profiles

随着DNA技术的发展和应用拓广,案件现场生物检材及其提取检验愈受重视、愈显重要性,近年来混合检材的DNA有效检出比例越来越高,混合STR分型的拆分与合理解释也益发成为DNA鉴定技术领域的研究热点与难点。本文主要针对当下国际主流的两款混合分型分析软件——STRmix和云算GPM进行比较研究,就两者的基本信息、理论、分析过程作比较,并结合实战应用和典型案例综合分析两者的异同,希冀为法医DNA工作者提供选用参考和使用依据,也希望能够推动国内混合STR分型分析的发展及与国际交流互鉴。

The ever-advancing DNA technology has been bringing the on-site biological evidential materials into more increasing attentions together with their collection and DNA testing,resulting in successful more detections of eligible STRs from mixed DNA evidential substances in recent years.Accordingly,more profiles of mixed STR are being encountered in forensic casework and waited for reasonal deconvolution and appropriate interpretation in routine task of DNA laboratory,leaving them becoming a hotspot and one of the greatest challenges for forensic DNA genotyping.STRmix and cloudcalculating GPM are presently the main-current two pieces of software to interpret mixed STRs of DNA,the most commonly used software worldwide,too.Discussions were here carried out on the two pieces of software about their similarities,differences,interpretation-dependent theories,analysis process and actual applications plus cases for exampling.References are intended to provide forensic DNA peers with the elucidated interpretation of mixed STR profiles and promotion of international exchange and cooperation.