摘要
[目的]确定利用马源乳酸菌制备阴道微生态制剂的制备工艺参数。[方法]以分离自蒙古马阴道的格氏乳杆菌作为制备微生态制剂的菌株,利用发酵学技术及响应面法,对菌株的发酵培养基、工艺参数及制剂的配方和工艺参数进行优化。[结果]乳糖和大豆胨对菌粉的生长性能影响较大,工艺参数中发酵液初始pH值为6.6,温度为40℃,菌种接种量为6%时,对菌粉的发酵性能影响较大。而后利用药物制备学技术及响应面法,对制剂的配方及工艺参数进行了优化,结果显示,菌粉与淀粉、微粉硅胶、微晶纤维素混合比例167.05∶1.61∶1,多功能药用机转速为20 r/min,角度为60°时所制备制剂的活菌数较其他工艺条件下高。[结论]确定了利用马源格氏乳杆菌制备阴道微生态制剂的关键工艺参数,为进一步探索应用活菌制备的微生态制剂预防和治疗马阴道感染提供参考。
[Objective]To determine the process parameters of production technology of a vaginal probiotic preparation using lactic acid bacteria of horse origin.[Method]A Lactobacillus gasseri strain isolated from Mongolian horse vagina,was used as a starter to product the probiotic preparation.Fermentation technology and response surface method were employed to optimize the strain′s fermentation medium and process parameters as well as the probiotic preparation′s formulation and process parameters.[Result]Lactose and soybean peptone had significant impacts on growth performance of the bacterial powder.When the initial pH value of the fermentation broth was set to 6.6,the temperature was set to 40℃,and the inoculation amount was set to 6%,the fermentation performance of the bacterial powder was largely influenced.Using pharmaceutical preparation technology and response surface method,the probiotic preparation′s formulation and process parameters were then adjusted.When the mixing ratio of bacterial powder to starch,micro-powder silica gel,and micro-crystalline cellulose was 167.05∶1.61∶1 and the rotating speed of multi-functional medicine machine was 20 r/min at 60°angle,the number of live bacteria in the obtained probiotic preparation was higher.[Conclusion]The critical process parameters for production of vaginal probiotic preparation with horse origined Lactobacillus gasseri were determined,providing a foundation for further explorations on the use of probiotic preparations made by live bacteria to prevent and treat horse vaginal infection.
作者
张昱
苏少锋
常嵘
白娜
张子玉
张泽云
张振奇
朱崇瑶
程超
ZHANG Yu;SU Shao-feng;CHANG Rong;BAI Na;ZHANG Zi-yu;ZHANG Ze-yun;ZHANG Zhen-qi;ZHU Chong-yao;CHENG Chao(College of Life Science and Technology,Jining Normal University,Jining District 012000,China;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)
出处
《畜牧与饲料科学》
2022年第1期8-15,共8页
Animal Husbandry and Feed Science
基金
2020年内蒙古自治区大学生创新创业训练计划项目“预防与治疗马体阴道炎症微生态制剂的制备”(202011427004)。
关键词
马
阴道
格氏乳杆菌
微生态制剂
制备工艺
horse
vagina
Lactobacillus gasseri
probiotic preparation
production technology