蓝莓花青素生物合成相关基因表达与分子进化分析

Expression and Molecular Evolution of Genes Related to Anthocyanin Biosynthesis in Blueberries

运用实时聚合酶链式反应方法,测定8个品种的蓝莓(包括6个高丛蓝莓:绿宝石、蓝美1号、海岸、莱格西、比洛克西、奥尼尔;2个兔眼蓝莓:括灿烂、园蓝)花青素合成途径中的VcCHS、VcCHI、VcF3H、VcDFR、VcANS结构基因及转录因子MYB基因相对表达量,分析其对花青素合成的调控作用。结果表明:VcCHS、VcCHI、VcF3H、VcDFR和VcANS基因对花青素的合成有调控作用,能够促进花青素的生成;但VcANR基因对花青素的合成有负调控作用,可使蓝莓花青素含量显著降低。MYB转录因子对花青素合成的调控作用相对复杂,与MYB转录因子的表达量有关。本研究对不同品种蓝莓花青素生物合成相关基因表达量和分子进化分析,以此研究优质的蓝莓品种在花青素方面的优势,为开发蓝莓花青素类功能性食品提供理论支撑。

In this study, we analyzed the relative expression levels of the structural genes VcCHS, VcCHI, VcF3 H, VcDFR and VcANS as well as the transcription factor MYB involved in the anthocyanin biosynthesis pathways in six cultivars of highbush blueberry including Emerald, Lanmei #1, Gulf Coast, Legacy, Biloxi, and O’neal and two cultivars of rabbiteye blueberry(i.e. Brightwell and Gardenblue) by real-time polymerase chain reaction(real-time PCR). The results showed that VcCHS, VcCHI, VcF3 H, VcDFR and VcANS could up-regulate the synthesis of anthocyanins and promote the production of anthocyanins. However, VcANR had a down-regulatory effect on anthocyanin synthesis, reducing the content of anthocyanins in blueberries. The regulatory effect of the transcription factor MYB on anthocyanin synthesis was relatively complex, which was related to its expression level. The results of this study can provide a foundation for further research on high-quality blueberry cultivars with respect to anthocyanins and for the development of functional foods containing blueberry anthocyanins.