非小细胞肺癌组织的TINCR水平及临床意义

Level and clinical significance of TINCR in non-small cell lung cancer tissues

目的探讨非小细胞肺癌(NSCLC)组织的TINCR水平及临床意义。方法收集本院2016年3月至2019年3月手术切除的89对NSCLC组织和癌旁组织,采用实时定量PCR(qPCR)检测上述组织的TINCR水平并采用受试者工作特征(ROC)曲线评估组织TINCR水平诊断NSCLC的效能,分层分析组织TINCR水平与NSCLC临床病理特征关系,结合随访数据进行生存分析并在Kaplan-Meier Plotter数据库中验证其预后意义。结果NSCLC组织的TINCR水平为3.714±1.369,低于癌旁组织的1.513±0.619(P<0.05);ROC曲线结果显示组织TINCR水平诊断NSCLC的曲线下面积为0.903(95%CI:0.856~0.951),且TINCR水平取2.401时约登指数最高(0.708),敏感度和特异度分别为79.78%和91.01%。组织TINCR水平与TNM分期、肿瘤大小、分化程度、淋巴结转移和总生存期(OS)有关(P<0.05),而与性别、年龄、吸烟史和病理类型无关(P>0.05)。44例低表达者的中位OS未达,优于高表达者的42.0(95%CI:31.569~52.431)个月,进一步多因素分析发现组织TINCR水平是OS的危险因素(HR=2.345,95%CI:1.658~4.805,P=0.019)。Kaplan-Meier Plotter数据库中验证发现,组织TINCR水平与首次进展后生存期(FP)、疾病进展后生存期(PPS)和OS有关,其中低水平患者的中位FP、OS和PPS分别为34.9、96.2和24.5个月,优于高水平患者18.1、67.0和10.8个月(P<0.05)。结论NSCLC组织中异常升高的TINCR水平参与NSCLC的发展,且TINCR高表达的NSCLC患者预后较差,该指标具有作为NSCLC筛查和预后预测标志物的潜力。

Objective To investigate the level and clinical significance of TINCR ubiquitin domain containing(TINCR)in non-small cell lung cancer(NSCLC)tissues.Methods Eighty-nine pairs of NSCLC tissues and adjacent tissues surgically removed in our hospital from March 2016 to March 2019 were collected.The TINCR level of the above tissues was detected by real-time quantitative PCR(qPCR),and the effectiveness of tissue TINCR level in diagnosing NSCLC was evaluated by receiver operating characteristic(ROC)curve.Relationship between tissue TINCR level and clinicopathological features was analyzed by stratification.The follow-up data were investigated for survival analysis,and its prognostic significance was verified in Kaplan-Meier Plotter database.Results The TINCR level of NSCLC tissues was 3.714±1.369,lower than 1.513±0.619 of adjacent tissues(P<0.05).ROC curve showed that the area under the curve of tissue TINCR level in the diagnosis of NSCLC was 0.903(95%CI:0.856-0.951),and when TINCR level was 2.401,the Yoden index was the highest(0.708)with the sensitivity of 79.78%and specificity of 91.01%.The TINCR level was related to TNM stage,tumor size,degree of differentiation,lymph node metastasis and overall survival(OS)(P<0.05),but not to gender,age,smoking history and pathological type(P>0.05).The median OS of 44 patients with low expression was not reached,better than 42.0(95%CI:31.569-52.431)months of those with high expression.Further multivariate analysis found that the tissue TINCR level was a risk factor for OS(HR=2.345,95%CI:1.658-4.805,P=0.019).It was verified in Kaplan-Meier Plotter database that tissue TINCR level was related to first progression(FP),post-progression survival(PPS)and OS.The median FP,OS and PPS of patients with low-level TINCR were 34.9,96.2 and 24.5 months,better than 18.1,67.0 and 10.8 months of patients with high-level TINCR(P<0.05).Conclusion The abnormally elevated TINCR level in NSCLC tissue is involved in the development of NSCLC,and the prognosis of NSCLC patients with high TINCR expression is poor.This index has the potential to be used as a marker for NSCLC screening and prognosis prediction.