当归精准煮散饮片指纹图谱建立及4种成分含量测定

Establishment of Fingerprint and Contents Determination of Four Components of Angelicae Sinensis Precision Boiled Powder Decoction Pieces

目的建立当归精准煮散饮片HPLC指纹图谱和4种成分的含量测定方法,比较当归精准煮散饮片与传统饮片主要化学成分含量差异,探讨两者之间对应量关系。方法采用HPLC测定当归精准煮散饮片与传统饮片中绿原酸、阿魏酸、洋川芎内酯Ⅰ和藁本内酯的含量,色谱柱为Robusta C18(250 mm×4.6 mm,5μm),流动相为乙腈(A)-0.2%磷酸溶液(B),流速1.0 mL/min,进样量20μL,柱温30℃,检测波长为280nm。结果建立了当归精准煮散饮片的特征指纹图谱,并指认出共有峰中4个主要峰,分别为绿原酸、阿魏酸、洋川芎内酯Ⅰ和藁本内酯;当归精准煮散饮片中绿原酸、阿魏酸、洋川芎内酯Ⅰ和藁本内酯含量高于当归传统饮片。通过对4种成分进行加权,比较当归精准煮散饮片与其传统饮片的综合得分,得到当归精准煮散饮片1 g相当于传统饮片1.26 g。结论当归传统饮片中4种成分含量均低于精准煮散饮片,本试验建立的指纹图谱及含量测定方法可为当归精准煮散饮片的质量控制及临床应用提供依据。

Objective To establish the HPLC fingerprint and the content determination method of four components of Angelicae Sinensis precision boiled powder decoction pieces;To compare the content difference of main chemical components between Angelica Sinensis precision boiled powder decoction pieces and traditional decoction pieces;To study the corresponding quantity relationship between them.Methods HPLC method was used to determine the contents of chlorogenic acid,ferulic acid,senkyunolideⅠand ligustilide in Angelicae Sinensis precision boiled powder decoction pieces and Angelicae Sinensis traditional decoction pieces.The chromatographic column was Robusta C18(250 mm×4.6 mm,5μm),and the mobile phase was acetonitrile(A)-0.2%phosphoric acid solution(B),with the flow rate of 1.0 mL/min.The injection volume was 20μL;column temperature was 30℃;the detection wavelength was 280 nm.Results The characteristic fingerprint of Angelicae Sinensis precision boiled powder decoction pieces was established,and four main common peaks,chlorogenic acid,ferulic acid,senkyunolideⅠand ligustilide were identified.The contents of chlorogenic acid,ferulic acid,senkyunolideⅠand ligustilide in Angelicae Sinensis precision boiled powder decoction pieces were higher than those in the traditional decoction pieces.By comprehensively weighting the four components and comparing the comprehensive scores of Angelicae Sinensis precision boiled powder decoction pieces with traditional decoction pieces,it is found that 1 g of Angelicae Sinensis precision boiled powder decoction pieces was equivalent to 1.26 g of traditional decoction pieces.Conclusion The contents of four components in Angelicae Sinensis traditional decoction pieces are lower than those in Angelicae Sinensis precision boiled powder decoction pieces,and the established fingerprint and content determination method can provide a basis for quality control and clinical application of Angelicae Sinensis precision boiled powder decoction pieces.