功能基因组学在苯并[a]芘毒性研究中的应用进展

Applications of functional genomics in research of benzo[a]pyrene toxicity:a review

苯并[a]芘(B[a]P)是一种公认的环境污染物,可导致肿瘤形成、免疫抑制、致畸性和激素效应等负面生物学效应。除了B[a]P暴露引起的遗传损伤外,越来越多的研究提示表观遗传学改变在化学诱导的致癌作用中起着重要作用。为更好地了解B[a]P表观遗传学改变,以及与遗传毒性终点的潜在联系,本文从功能基因组学技术、人类基因组表达调控研究、DNA序列变异性研究、模式生物研究和生物信息学研究等方面对功能基因组学在B[a]P毒性研究中的应用进行综述,为B[a]P暴露引起的健康损伤的防治及功能基因组学在其他环境污染物中的毒性机制研究提供依据。

Benzo[a]pyrene(B[a]P)is a well-recognized environmental pollutant.Exposure to B[a]P elicits many adverse biological effects,including tumorigenesis,immunosuppression,teratogenicity,and hormonal effects.In addition to B[a]P exposure-induced genetic damages,a growing number of studies demonstrate that epigenetic changes play an important role in chemically induced carcinogenesis.In order to provide better understanding of epigenetic changes of B[a]P and their potential association with genotoxic endpoints,this review summarizes the advances in the applications of function⁃al genomics in the research of B[a]P toxicity,including functional genomics techniques,regulation of human genome ex⁃pression,DNA sequence variability,model organisms research,and bioinformatics studies,so as to provide insights into the management of B[a]P exposure-induced health injuries and use of genomics techniques to unravel the mechanisms underlying the toxicity of other environmental pollutants.