早春栽培的印度南瓜果实淀粉积累相关酶的活力

Dynamic changes of fruit starch accumulation-related enzyme activities in early spring cultivated Cucurbita maxima

为选育果实淀粉含量高、适于早春日光温室栽培的印度南瓜,以Agol、雪2父、S8844、无名绿4份高代自交系种质为试材,研究果实发育期间(授粉后14、21、28、35、42 d)淀粉含量动态变化及其与AGPase、GBSS、SSS、SBE、α-淀粉酶、β-淀粉酶等酶活力的相关性。结果表明,果实发育42 d后,高淀粉含量种质S8844和无名绿的干物质含量(w,后同)分别为26.1%、33.0%,淀粉含量分别为85.2%(干质量)、87.1%(干质量)。与淀粉积累有关的AGPase、GBSS、SBE等酶活力均高于低淀粉含量的2个种质(Agol、雪2父)。在进行适于早春日光温室栽培的印度南瓜品种选育时,可选择果实发育前期的APGase和SBE酶活力作为高淀粉含量种质的筛选指标之一,为高品质印度南瓜种质早期筛选鉴定提供一定的参考依据。

In order to breed Cucurbita maxima with high starch content and suitable for greenhouse cultivation in early spring,the dynamic changes of starch accumulation and correlation relationship with the activities of AGPase,GBSS,SSS,SBE,α-amylase,β-amylase were studied in the four inbred lines Agol,Xue2 male,S8844 and Wuminglü.The results showed that after 42 d of fruit development,the dry matter contents of S8844 and Wuminglüwere 26.1%and 33.0%,and the starch contents were 85.2%(Dry weight)and 87.1%(Dry weight),respectively.The enzyme activities of AGPase,GBSS and SBE were higher than those of the other two germplasms.The enzyme activities of SBE and AGPase in the early stage of fruit development could be selected as one of the biochemical indexes.The study could provide a theoretical basis for Cucurbita maxima breeding in early spring greenhouse.