二肽基肽酶-4在瘢痕疙瘩中的表达情况研究

Study on the Expression of Dipeptidyl Peptidase-4 in Keloids

目的:探讨二肽基肽酶-4(DPP4)在瘢痕疙瘩中的表达情况。方法:分别获取切除术后的瘢痕疙瘩组织及正常皮肤组织,然后通过蛋白免疫印迹方法、定量聚合酶链反应,比较二者DPP4基因在蛋白和mRNA水平上表达的差异。结果:与正常皮肤组织相比,DPP4蛋白在瘢痕疙瘩组织中的表达高于正常皮肤,差异有统计学意义(P<0.05),DPP4基因在mRNA水平上的表达高于正常皮肤(P<0.05)。结论:DPP4在瘢痕疙瘩中的表达上升,DPP4可能在促进成纤维细胞活化、纤维化形成方面有一定作用。

Objective To explore the expression of dipeptidyl peptidase-4 in keloids.Methods The keloid tissue and normal skin tissue after resection were obtained,and then the differences in the expression of DPP4 gene between the two were compared by Western blotting and quantitative polymerase chain reaction.Results Compared with normal skin tissue,the expression of DPP4 protein in keloid tissue was higher than that in normal skin,and the difference was statistically significant(P<0.05).The expression of DPP4 gene at mRNA level was higher than that in normal skin(P<0.05).Conclusion The expression of DPP4 in keloids is increased.DPP4 may play a role in promoting fibroblast activation and fibrosis formation.