食管癌患者血清miR-3653-3p和miR-330-3p的作用及临床意义

Role and clinical significance of serum miR-3653-3p and miR-330-3p in patients with esophageal cancer

目的观察血清miR-3653-3p和miR-330-3p表达水平对食管癌诊断和预后预测的临床价值。方法选取2015年1月至2017年6月在该院行手术治疗的食管癌患者109例作为食管癌组,同期在该院行手术治疗的食管良性病变患者(65例)和体检健康者(30例)分别作为食管良性病变组和对照组。采用实时荧光定量PCR检测血清miR-3653-3p和miR-330-3p水平,采用受试者工作特征(ROC)曲线分析miR-3653-3p和miR-330-3p水平对食管癌的诊断效能和预后预测价值。结果食管癌组患者血清miR-3653-3p水平低于食管良性病变组和对照组(P<0.01),食管良性病变组血清miR-3653-3p水平低于对照组(P<0.01);食管癌组患者血清miR-330-3p水平高于食管良性病变组和对照组(P<0.01),食管良性病变组miR-330-3p水平高于对照组(P<0.01)。ROC曲线分析显示,血清miR-3653-3p和miR-330-3p水平对食管癌有较高的诊断效能,联合检测的曲线下面积(AUC)高于miR-365403-13p、miR-330-3p单独检测。3年随访存活组血清miR-3653-3p水平明显高于死亡组(P<0.01),而血清miR-330-3p水平明显低于死亡组(P<0.01)。血清miR-3653-3p和miR-330-3p水平对食管癌患者3年内出现死亡具有较高的预测价值,且联合检测的AUC高于miR-3653-3p、miR-330-3p单独检测。结论miR-3653-3p和miR-330-3p在食管癌中的作用类似抑癌基因和促癌基因,血清miR-3653-3p和miR-330-3p联合检测在食管癌诊断和预测3年内死亡方面有重要价值。

Objective To observe the clinical value of serum miR-3653-3p and miR-330-3p expression levels in the diagnosis and prognosis of esophageal cancer.Methods From January 2015 to June 2017,109 patients with esophageal cancer who underwent surgical treatment in the hospital were selected as esophageal cancer group.Meanwhile,patients with benign esophageal disease who underwent surgical treatment(65 cases)in the hospital were selected as benign esophageal disease group,and healthy subjects(30 cases)were selected as control group respectively.Real-time fluorescence quantitative PCR was used to detect serum miR-3653-3p and miR-330-3p levels,and receiver operating characteristic(ROC)curve was used to analyze the diagnostic efficacy and prognostic value of miR-3653-3p and miR-330-3p levels for esophageal cancer.Results The serum miR-3653-3p level in esophageal cancer group was lower than that in benign esophageal disease group and control group(P<0.01),and it in benign esophageal disease group was lower than that in control group(P<0.01).The serum miR-330-3p level in esophageal cancer group was higher than those in benign esophageal disease group and control group(P<0.01),and it in benign esophageal disease group was higher than that in control group(P<0.01).ROC curve analysis showed that serum miR-3653-3p and miR-330-3p levels had higher diagnostic efficacy for esophageal cancer,and the area under curve(AUC)of combined detection was higher than those of miR-365403-13p and miR-330-3p alone.After 3-year follow-up the serum miR-3653-3p level in the survial group was significantly higher than that in the death group(P<0.01),while the serum miR-330-3p level was significantly lower than that in the death group(P<0.01).Serum miR-3653-3p and miR-330-3p levels had higher diagnostic efficacy for esophageal cancer,and the AUC of combined detection was higher than that of miR-3653-3p and miR-330-3p alone.Serum miR-3653-3p and miR-330-3p levels had a high predictive value for the death of esophageal cancer patients within 3 years,and the AUC of combined detection was higher than that of miR-3653-3p and miR-330-3p alone.Conclusion The roles of miR-3653-3p and miR-330-3p in esophageal cancer are similar to tumor suppressor genes and tumor-promoting genes.The combined detection of serum miR-3653-3p and miR-330-3p has important clinical value in the diagnosis and prediction of esophageal cancer death within 3 years.