两株无花果活性内生细菌的分离鉴定及生物学特征研究

Research on Biological Characteristics and Isolation Identification of Active Endophytic Fungi from Two Ficus Carica

采用LB平板从无花果果浆中分离出19株内生细菌,用金黄色葡萄球菌、大肠杆菌和变形杆菌筛选得到两株活性内生细菌,通过菌落及形态观察、分子生物学技术对其进行分类鉴定,确定为芽孢杆菌属细菌,生理生化验证后命名为Bacillus WHG3251、Bacillus strain BF3252,对两株无花果活性内生芽孢杆菌芽孢形成时间、耐碱性、耐盐性和产淀粉酶能力等生物学特征进行研究。结果表明:两株内生活性细菌产芽孢时间分别为25h和26h,最适生长pH值为7.0,有一定耐碱性、耐盐性和产淀粉酶的能力,两株芽孢杆菌在耐碱性、耐盐性和产淀粉酶能力等方面有较大差异。

In this study,19 endophytic fungi were isolated from ficus carica.Using LB plate,two active endophytic fungi were screened by Staphylococcus aureus,Escherichia coli,P.vulgaris,they were classified and identified by observation of colony and molecular biology techniques,they were identified as bacillus,they’re named Bacillus WHG3251,Bacillus strain BF3252 after physiological and biochemical verification,the biological characteristics were studied of two active endophytic fungi about spore formation time,alkali tolerance,salt tolerance and amylase production capacity.The results show:the spore-producing time of the two active endophytic fungi was 25h and 26h,the optimum growth pH is 7.0,there is a certain alkali resistance,salt resistance and amylase production ability,there were significant differences in alkali tolerance,salt tolerance and amylase production between the two strains of bacillus.