谷胱甘肽包覆Cu⁃In⁃Zn⁃S量子点的一锅法水相合成及细胞成像应用

One⁃Pot Aqueous Synthesis and Cell Labeling Application of Glutathione Capped Cu⁃In⁃Zn⁃S Quantum Dots

以生物相容性的谷胱甘肽(GSH)为封端配体和稳定剂,采用一锅法在水相介质中直接合成了高荧光的Cu‑In‑Zn‑S(CIZS)量子点。系统地研究了反应时间、pH值、前驱体配比等实验参数对合成结果的影响,并采用UV‑Vis、荧光光谱、透射电镜、粉末X射线衍射和FT‑IR等技术对所得CIZS量子点的光学性质和结构进行了表征。结果表明,在优化条件下,GSH包覆的CIZS量子点具有优异的光致发光性能、较窄的尺寸分布和较好的生物相容性。同时,CIZS量子点成功用于MDA‑MB‑231细胞荧光成像,并发出明亮的红色荧光。

We report a one‑pot method to directly synthesize highly luminescent Cu‑In‑Zn‑S(CIZS)quantum dots(QDs)in aqueous media by using bio‑compatible glutathione(GSH)as capping ligand and stabilizer.The influences of various experimental variables,including reaction time,pH value,and precursor ratio,have been systematically investigated.The optical features and structure of the obtained CIZS QDs have been characterized by UV‑Vis and fluorescence spectroscopy,transmission electron microscope,powder X‑ray diffraction,and FT‑IR.As a result,the stable GSH capped CIZS QDs exhibited excellent photoluminescence emission properties,narrow size distribution and excellent biocompatibility under optimum experimental conditions.In addition,as‑prepared CIZS QDs were successfully used for fluorescence imaging of MDA‑MB‑231 cells and emitted bright red fluorescence.