循环肿瘤细胞富集技术研究进展

Progress on the enrichment technology of circulating tumor cells

循环肿瘤细胞(CTC)是从原发性或转移性肿瘤部位脱落至循环系统的极少量的肿瘤细胞,与肿瘤的转移密切相关。从血液样本中分离出CTC并对其进行后续分析,对了解细胞水平的肿瘤转移和疾病临床管理都至关重要。由于CTC在血液中含量极少,难以被富集,因此限制了其临床应用。近年来,随着富集分离技术的不断进步,CTC作为肿瘤生物标志物对疾病进行预后评估逐渐成为研究热点。文章综述了CTC分离技术的最新进展,介绍了该类技术的临床应用并讨论其优缺点、未来展望和技术难题,力求为后续开展相关研究提供参考。

Circulating tumor cells(CTC)are a few tumor cells that are shed from the site of a primary or metastatic tumor into the circulatory system.As such,the isolation of CTC from blood samples and their subsequent analysis are of importance not only for understanding cancer metastasis at the cellular level but also for clinical management of the disease.Since the content of CTC in blood is too scarce to be enriched,which has become the bottleneck restricting its clinical application.In recent years,with the development of separation and enrichment techniques,CTC as a tumor biomarker for disease prognosis evaluation has become a hot topic.This review aims to review the latest advances of CTC isolation technology in cancer diagnosis,introduces the application examples of this technology,and discusses its advantages and disadvantages,prospects and technical challenges,to provide a reference for follow-up research.