摘要
目的:探讨高住低训(HiLo)对大鼠骨骼肌中低氧诱导因子(HIF-1α)和血管内皮因子(VEGF)蛋白表达的影响。方法:40只SD大鼠随机分为常氧安静组(C)、常氧训练组(E)、低氧安静组(L)和高住低训组(H)。实验选择模拟2500m海拔低氧环境,以20m/min的速度,6次/周,40min/d进行跑台训练。采用HE染色法观察高住低训对大鼠骨骼肌形态影响,RT-PCR法检测,半定量分析大鼠腓肠肌HIF-1αmRNA和VEGF m RNA表达,Western Blot检测,Image分析大鼠腓肠肌HIF-1α和VEGF蛋白表达。结果:H组HIF-1αm RNA和VEGF m RNA表达量显著升高(P<0.05),H组HIF-1α和VEGF蛋白表达显著升高(P<0.05)。结论:高住低训诱导HIF-1αmRNA和VEGF m RNA表达,高住低训对大鼠腓肠肌起到保护作用,其机制可能是通过上调腓肠肌HIF-1α和VEGF蛋白表达。
Objective:To investigate the effects of HiLo on the expression of hypoxia induction factor(HIF-1)and vascular endothelial factor(VEGF)in skeletal muscle of rats. Methods:40 SD rats were randomly divided into 4 groups:normal oxygen quiet group(C),normal oxygen training group(E),low oxygen quiet group(L),and high occupancy low training group(H). In the experiment,2500 m altitude low oxygen environment was simulated and treadmill training was carried out at a speed of 20 m/min,6 times/week and 40 min/d. HE staining was used to observe the effects of high residence and low training on skeletal muscle morphology in rats. Rt-pcr was used to detect the mRNA expression of HIF-1 and VEGF in rats’ gastrocenteric muscle. Western Blot was used to detect the protein expression of HIF-1 and VEGF in rats’ gastrocenteric muscle. Results:The m RNA expression levels of HIF-1 and VEGF were increased in Group H(P < 0.05),while the protein expressions of HIF-1 and VEGF were increased in group H. Conclusion:HiF-1 m RNA and VEGF mRNA expression were induced by hiF-1 and VEGF mRNA expression. Hif-1 mRNA and VEGF mRNA expression were induced by hiF-1 mRNA expression. Hif-1 mRNA and VEGF mRNA expression were induced by hiF-1 mRNA expression.
作者
李晨
李英英
李雯婷
LI Chen;LI Yingying;LI Wenting(Physical Education College of Xinjiang Normal University,Urumqi 830054,Xinjiang,China)
出处
《辽宁体育科技》
2022年第1期79-84,共6页
Liaoning Sport Science and Technology