生与炒酸枣仁配伍对状态性焦虑模型大鼠行为学及单胺类和氨基酸类神经递质表达的影响

Effects of Compatibility of Raw and Fried Jujube on Ethology and Expressions of Monoamine and Amino Acid Neurotransmitters in State Anxiety Model Rats

目的:阐明生与炒酸枣仁配伍拮抗大鼠状态性焦虑作用的神经递质机制。方法:SD大鼠随机分为5组,空白对照组、模型组、生与炒酸枣仁配伍组、炒酸枣仁对照组、地西泮对照组(3.6 mg·kg^(-1))。经4 d条件性恐惧电刺激复制状态性焦虑动物模型。造模之后,连续灌胃给药10 d。用在体微透析技术和电化学检测器(HPLC-ECD)联合使用测定海马和外侧杏仁核(LA)中去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)的含量。用在体微透析技术和高效液相色谱与OPA柱前衍生技术(HPLC-OPA)测定海马和外侧杏仁核中Glu、GABA的含量。结果:在LA脑区中,与空白对照组相比,模型组DA、NE、5-HT、Glu、GABA含量显著增加(P<0.05,P<0.01);与模型组相比:生与炒酸枣仁配伍组DA、5-HT、Glu含量显著减少(P<0.05,P<0.01);与地西泮对照组相比,生与炒酸枣仁配伍组DA、5-HT、NE、Glu、GABA含量无显著差异。在海马脑区中,与空白对照组相比,模型组大鼠DA、NE、5-HT、Glu含量显著增加,GABA含量显著减少(P<0.05,P<0.01);与模型组相比,生与炒酸枣仁配伍组大鼠DA、NE、5-HT、Glu含量均显著性减少(P<0.05,P<0.01);与地西泮对照组相比,生与炒酸枣仁配伍组DA、NE、GABA含量无显著性差异;5-HT、Glu含量显著性减少(P<0.05,P<0.01)。结论:生与炒酸枣仁配伍可以拮抗焦虑,且作用效果优于炒酸枣仁,生与炒酸枣仁配伍拮抗焦虑的机制可能与调节海马、LA脑区中单胺类及氨基酸类神经递质含量有关。

Objective:To clarify the neurotransmitter mechanism of the compatibility of raw and fried jujube in antagonizing state anxiety in rats.Methods:SD rats were randomly divided into the blank control group,the model group,the compatibility group of raw and fried jujube,the fried jujube group and the Diazepam group(3.6 mg·kg^(-1)).The animal model of state anxiety was replicated by 4 days of conditioned fear electrical stimulation.After 10 days of administration,the contents of NE,DA and 5-HT of hippocampus and lateral amygdala(LA)were determined by the combined use of in vivo microdialysis technology and electrochemical detector(HPLC-ECD).In vivo microdialysis technology,high performance liquid chromatography and OPA pre-column derivatization technology(HPLC-OPA)were used to determine the contents of Glu and GABA in hippocampus and LA.Results:In the LA brain area,compared to those in the blank control group,the contents of DA,NE,5-HT,Glu and GABA increased significantly in the model group(P<0.05,P<0.01);compared to those in the model group,the contents of DA,5-HT and Glu significantly reduced in the compatibility group of raw and fried jujube(P<0.05,P<0.01);there were no statistical differences in the contents of DA,5-HT,NE,Glu and GABA between the Diazepam group and the compatibility group of raw and fried jujube.In hippocampus brain area,compared to those in the blank control group,the contents of DA,NE,5-HT and Glu significantly increased,and the content of GABA significantly decreased in the model group(P<0.05,P<0.01);compared to those in the model group,the contents of DA,NE,5-HT and Glu significant reduced in the compatibility group of raw and fried jujube(P<0.05,P<0.01);compared to those in the Diazepam group,the contents of 5-HT and Glu significantly reduced in the compatibility group of raw and fried jujube(P<0.05,P<0.01),but there were no statistical differences in the contents of DA,NE and GABA between the two groups.Conclusion:The compatibility of raw and fried jujube can antagonize anxiety,and the effect is better than that of fried jujube.The mechanism of antagonizing anxiety by compatibility of raw and fried jujube may be related to the regulation of monoamine and amino acid neurotransmitters in hippocampus and LA brain regions.