SPHK1、VEGF在胃癌中的表达及其与临床病理特征和预后的关系

Expression of SPHK1 and VEGF in gastric carcinoma and its relationship with clinicopathological features and prognosis

目的探讨鞘氨醇激酶1(SPHK1)与血管内皮生长因子(VEGF)在人胃癌组织中的表达及其与临床病理特征和预后的关系。方法采用免疫组化SP法检测SPHK1与VEGF蛋白在178例胃癌组织及其162份癌旁组织中的表达情况,使用Kaplan-Meier Plotter数据库了解SPHK1、VEGF对胃癌患者生存时间的影响。结果 SPHK1在胃癌组织中高表达率为75.3%,明显高于癌旁组织的32.1%(P<0.05);SPHK1表达与患者的淋巴结转移、脉管侵犯及T分期有关(P<0.05),与患者的年龄、性别、分化程度、神经侵犯、远处转移及肿瘤大小均无明显关系(P>0.05)。VEGF在胃癌组织中的高表达率为62.9%,明显高于癌旁组织中的44.4%(P<0.05);VEGF表达与患者淋巴结转移、脉管侵犯、神经侵犯、T分期及远处转移有关(P<0.05),与患者的年龄、性别、分化程度及肿瘤大小无明显关系(P>0.05)。SPHK1与VEGF在胃癌组织中的表达呈正相关(r=0.207,P<0.05)。SPHK1高表达组的中位总生存期为25.0个月,显著短于SPHK1低表达组的57.1个月(HR=1.56,95%CI:1.29~1.89,P=0.000);VEGF高表达组的中位总生存期为20.5个月,显著短于VEGF低表达组的33.6个月(HR=1.49,95%CI:1.23~1.81,P=0.000)。结论 SPHK1可能与VEGF共同调控胃癌的生物学过程。

Objective To investigate the expression of sphingosine kinase 1(SPHK1) and vascular endothelial growth factor(VEGF) in human gastric carcinoma tissue and its relationship with clinicopathological features and prognosis.Methods The expression of SPHK1 and VEGF proteins in 178 gastric carcinoma tissues and 162 paracancer tissues were detected by immunohistochemical SP method.The effects of SPHK1 and VEGF on the survival time of gastric carcinoma patients were understood by Kaplan-Meier Plotter database.Results The high expression rate of SPHK1 in gastric carcinoma tissues was 75.3%,which was significantly higher than 32.1% in paracancer tissues(P <0.05);SPHK1 expression was associated with lymph node metastasis,vascular invasion and T stage(P <0.05),but had no significant relationship with age,gender,differentiation degree,nerve invasion,distant metastasis and tumor size(P>0.05).The high expression rate of VEGF in gastric carcinoma tissues was 62.9%,which was significantly higher than 44.4% in paracancer tissues(P<0.05);VEGF expression associated with lymph node metastasis,vascular invasion,nerve invasion,T stage and distant metastasis(P<0.05),but had no significant relationship with age,gender,differentiation degree and tumor size(P>0.05).There was a positive correlation between the expression of SPHK1 and VEGF in gastric carcinoma tissues(r=0.207,P<0.05).The median overall survival of SPHK1 high expression group was 25.0 months,which was significantly shorter than 57.1 months of SPHK1 low expression group(HR=1.56,95%CI:1.29-1.89,P=0.000);the median overall survival of VEGF high expression group was 20.5 months,which was significantly shorter than 33.6 months of VEGF low expression group(HR=1.49,95%CI:1.23-1.81,P=0.000).Conclusion SPHK1 may co-regulate the biological process of gastric carcinoma with VEGF.