仙方活命饮免煎颗粒醋调外敷箍围法治疗肛周炎性肿块的机制初探

A study on the mechanism of external application of Xianfang Huoming Yin on perianal inflammatory mass

目的:通过观察仙方活命饮对肛周炎性肿块的主要致病菌——大肠埃希菌及肺炎克雷伯菌的体外抑菌作用,探讨仙方活命饮免煎颗粒醋调外敷箍围法治疗肛周炎性肿块的可能机制。方法:分别以仙方活命饮饮片水煎剂、水溶免煎颗粒、醋溶免煎颗粒为实验组,左氧氟沙星氯化钠注射液为对照组,通过最低抑菌浓度(Minimum Inhibitory Concentration,MIC)检测及牛津杯实验观察两种治疗方法对大肠埃希菌及肺炎克雷伯菌的抑菌效果及MIC值。结果:仙方活命饮饮片水煎剂及水溶仙方活命饮免煎颗粒对上述两菌均无抑菌作用;相较于肺炎克雷伯菌,仙方活命饮片饮水煎剂可以减缓大肠埃希菌的生长速度;醋溶仙方活命饮免煎颗粒可抑制大肠埃希菌及肺炎克雷伯菌的生长,MIC值分别为20 mg/mL及40 mg/mL。结论:醋调外敷仙方活命饮免煎颗粒箍围治疗肛周炎性肿块的机制可能与改善致病菌生长的微环境、调节局部酸碱度、提高创面局部免疫功能、改善局部血液循环等有关。

Objective:To study the antibacterial effects in vitro of Xianfang Huoming Yin(仙方活命饮)on escherichia coli and klebsiella pneumoniae,and explore the possible mechanism of Xianfang Huoxing Yin in therapy of encircling lesion with drugs on the perianal inflammatory mass.Methods:Xianfang Huoming Yin for drinking,water-soluble free-decocted granules,and vinegar-soluble decoctedfree granules were used in the experimental group,and levofloxacin injection was used in the control group.The MIC test and Oxford Cup experiment were used to observe the effects on antibacterial and minimum inhibitory concentration.Results:Neither Xianfang Huoming Yin decoction pieces nor water-soluble free-decocted granules have antibacterial effects on the above two bacteria.Compared with klebsiella pneumonia,Xianfang Huoming Yin for drinking can slow down the growth rate of escherichia coli;vinegar-soluble decocted-free granules of Xianfang Huoming Yin can inhibit the growth of escherichia coli and klebsiella pneumoniae,with MIC values of 20 mg/mL and 40 mg/mL,respectively.Conclusion:The mechanism of vinegar-adjusted external application of Xianfang Huoming Yin decoction-free granules on perianal inflammatory masses may be related to the improvement of the microenvironment for the growth of pathogenic bacteria,the regulation of local pH,the improvement of local immune function of the wound,and the improvement of local blood circulation.