基于网络药理学对白鲜皮--地肤子治疗尿毒症性瘙痒症机制研究

A study on the mechanism of Dictamnus dasycarpus-Kochia scoparia on uremic pruritus based on network pharmacology

目的:基于网络药理学方法分析白鲜皮-地肤子治疗尿毒症性瘙痒症(非透析)的药理机制,为新药研发及临床拓展运用提供参考。方法:通过中药系统药理学数据库和分析平台获取白鲜皮、地肤子的主要化学成分及其靶点,根据药代动力学筛选中药活性组分;通过GeneCards数据库获取尿毒症性瘙痒症主要靶点,构建“药物-成分-靶点”网络图;利用String平台进行蛋白质相互作用分析,构建蛋白质相互作用网络。采用Metascape平台分析其参与的生物过程及通路。结果:白鲜皮-地肤子调治尿毒症性瘙痒症的核心活性成分为槲皮素、山柰酚、木犀草素等,核心靶点有白细胞介素-6、血管内皮生长因子、肿瘤坏死因子、肿瘤抑制基因53、基质金属蛋白酶9、前列腺素氧化环化酶2等。白鲜皮-地肤子治疗尿毒症性瘙痒症的生物学通路主要作用于缺氧诱导因子-1信号通路和血管内皮生长因子信号通路等通路。结论:本研究初步揭示了白鲜皮-地肤子治疗尿毒症性瘙痒症的多成分、多靶点、多通路的作用机制,为此药对的临床开发利用提供基础。

Objective:To analyze the pharmacological mechanism of Dictamnus dasycarpus-Kochia scoparia on uremic pruritus(nondialysis)based on network pharmacology,and to provide reference for new drug development and clinical application.Methods:The main chemical components and their targets of Dictamnus dasycarpus and Kochia scoparia were obtained through the TCMSP database,and the active components of traditional Chinese medicine were screened according to ADME;the main targets of dyslipidemia were obtained through the GeneCards database.A“drug-component-target”network diagram was constructed;by the String platform,protein-protein interactions were analyzed.A PPI network was constructed.The Metascape platform was used to analyze the biological processes and pathways it participated in.Results:The core active ingredients of Dictamnus dasycarpus-Kochia scoparia on uremic pruritus were quercetin,kaempferol,luteolin,etc..The core targets were IL6,VEGFA,TNF,TP53,MMP9,PTGS2,etc..The biological pathway of Dictamnus dasycarpus-Kochia scoparia on uremic pruritus mainly acts on the hypoxia-inducible factor-1 signaling pathway and the vascular endothelial growth factor signaling pathway.Conclusion:This study initially revealed the multi-component,multi-target,and multi-path mechanism of Dictamnus dasycarpus-Kochia scoparia on uremic pruritus,and provided the basis for the clinical development and utilization of this drug pair.